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PMID: 4341522 Published · ppublish English Journal Article

Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis.

Applied microbiology ·Vol. 24 ·No. 1 ·1972-07-00 ·Pages 96-100

Jeansson S

Abstract

A method has been elaborated to differentiate between herpes simplex type 1 and type 2 viruses by immunoelectroosmophoresis. With rabbit immune sera cross-absorbed with heterologous virus antigen, a distinct difference was shown between the two virus types. Herpes simplex type 1 virus tested against cross-absorbed type 1 antiserum gave two precipitin lines. Herpes simplex type 2 virus gave one precipitin line when tested against cross-absorbed homologous serum. When the viral antigens were tested against cross-absorbed heterologous immune sera, no or only very weak precipitin reactions were observed. The test is easy and rapid, requires relatively small quantities of antigen and antibody, and is suitable for typing of herpes simplex virus in diagnostic routine work.

MeSH Terms
Absorption Animals Antigens, Viral/analysis Cell Line/microbiology Chick Embryo Cricetinae Culture Techniques Haplorhini Immune Sera Immunoelectrophoresis Kidney Methods Rabbits/immunology Serotyping Simplexvirus/classification,growth & development Virus Cultivation
Chemicals
Antigens, Viral Immune Sera
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jeansson S
References (21)
21 references, click to expand
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Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.24.1.96-100.1972
Published
1972-07-00
Pages
96-100
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC380553
Subset
IM
External Links
PubMed source DOI: 10.1128/am.24.1.96-100.1972 PMC Full Text
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