Home LiteratureArticle Details
PMID: 4342212 Published · ppublish English Journal Article

Some properties of pyruvate and 2-oxoglutarate oxidation by blowfly flight-muscle mitochondria.

The Biochemical journal ·Vol. 127 ·No. 1 ·1972-03-00 ·Pages 271-83

Hansford RG

Abstract

1. High rates of state 3 pyruvate oxidation are dependent on high concentrations of inorganic phosphate and a predominance of ADP in the intramitochondrial pool of adenine nucleotides. The latter requirement is most marked at alkaline pH values, where ATP is profoundly inhibitory. 2. Addition of CaCl(2) during state 4, state 3 (Chance & Williams, 1955) or uncoupled pyruvate oxidation causes a marked inhibition in the rate of oxygen uptake when low concentrations of mitochondria are employed, but may lead to an enhancement of state 4 oxygen uptake when very high concentrations of mitochondria are used. 3. These properties are consistent with the kinetics of the NAD-linked isocitrate dehydrogenase (EC 1.1.1.41) from this tissue, which is activated by isocitrate, citrate, ADP, phosphate and H(+) ions, and inhibited by ATP, NADH and Ca(2+). 4. Studies of the redox state of NAD and cytochrome c show that addition of ADP during pyruvate oxidation causes a slight reduction, whereas addition during glycerol phosphate oxidation causes a ;classical' oxidation. Nevertheless, it is concluded that pyruvate oxidation is probably limited by the respiratory chain in state 4 and by the NAD-linked isocitrate dehydrogenase in state 3. 5. The oxidation of 2-oxoglutarate by swollen mitochondria is also stimulated by high concentrations of ADP and phosphate, and is not uncoupled by arsenate.

MeSH Terms
Adenine Nucleotides/metabolism Adenosine Diphosphate Adenosine Monophosphate/metabolism Alcohol Oxidoreductases/metabolism Animals Arsenic Bicarbonates Calcium Chloride Citrates Diptera/metabolism Enzyme Activation Glutarates/metabolism Hydrogen-Ion Concentration Isocitrates Kinetics Mitochondria, Muscle/metabolism NAD Oxidation-Reduction Oxygen Consumption Phosphates/metabolism Proline Pyruvates/metabolism Temperature
Chemicals
Adenine Nucleotides Bicarbonates Citrates Glutarates Isocitrates Phosphates Pyruvates NAD Adenosine Monophosphate Adenosine Diphosphate Proline Alcohol Oxidoreductases Calcium Chloride Arsenic
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hansford R G
References (32)
32 references, click to expand
  1. Regulation of metabolism in working muscle in vivo. II. Concentrations of adenine nucleotides, arginine phosphate, and inorganic phosphate in insect flight muscle during flight.
    J Biol Chem. 1966 Feb 10;241(3):632-4 PMID: 4222257
  2. Pyruvate oxidation and the permeability of mitochondria from blowfly flight muscle.
    Science. 1966 Oct 14;154(3746):268-70 PMID: 5914759
  3. Unspecific permeation and specific exchange of adenine nucleotides in liver mitochondria.
    Biochim Biophys Acta. 1965 Jun 15;104(1):312-5 PMID: 5840415
  4. Lactic and alpha-glycerophosphate dehydrogenases in insects.
    J Gen Physiol. 1957 May 20;40(5):779-90 PMID: 13428988
  5. Changes of total water and sucrose space accompanying induced ion uptake or phosphate swelling of rat liver mitochondria.
    Biochem J. 1968 Feb;106(3):759-66 PMID: 5639931
  6. On the importance of alpha-glycerophosphate dehydrogenase in glycolysing insect muscle.
    Biochim Biophys Acta. 1958 Jun;28(3):660-1 PMID: 13560433
  7. Mechanism of phosphorylation in the respiratory chain.
    Nature. 1953 Nov 28;172(4387):975-8 PMID: 13111237
  8. Control of the tricarboxylate cycle and its interactions with glycolysis during acetate utilization in rat heart.
    Biochem J. 1970 May;117(4):677-95 PMID: 5449122
  9. Distribution of phosphorus compounds in blowfly thoracic muscle.
    Biochem J. 1959 Jan;71(1):176-85 PMID: 13628550
  10. Pyruvate oxidation and the permeability of housefly sarcosomes.
    Biochem J. 1964 Oct;93(1):128-36 PMID: 5838089
  11. The effect of Ca2+ on the oxidation of glycerol phosphate by blowfly flight-muscle mitochondria.
    Biochem Biophys Res Commun. 1967 Jun 23;27(6):686-92 PMID: 4964598
  12. Energy-linked alteration of the permeability of heart mitochondria to chloride and other anions.
    Biochemistry. 1970 Feb 17;9(4):697-707 PMID: 5417390
  13. The control of the oxidation of proline by isolated flight muscle mitochondria.
    J Biol Chem. 1970 Mar 10;245(5):991-4 PMID: 5417269
  14. Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria.
    Eur J Biochem. 1969 Feb;7(4):471-84 PMID: 5776240
  15. Quantitative correlation between the distribution of anions and the pH difference across the mitochondrial membrane.
    Eur J Biochem. 1970 Dec;17(2):230-8 PMID: 5500391
  16. The respiratory activity and permeability of housefly sarcosomes.
    Biochem J. 1962 Feb;82:362-71 PMID: 13867712
  17. Adenine nucleotide translocation of mitochondria. 1. Specificity and control.
    Eur J Biochem. 1968 Oct 17;6(1):66-79 PMID: 5725814
  18. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.
    Biochemistry. 1968 Nov;7(11):4030-4 PMID: 4972613
  19. Alpha-keto acid dehydrogenase complexes. XI. Comparative studies of regulatory properties of the pyruvate dehydrogenase complexes from kidney, heart, and liver mitochondria.
    Proc Natl Acad Sci U S A. 1969 Sep;64(1):227-34 PMID: 4312751
  20. Metabolite transport in mitochondria: an example for intracellular membrane function.
    Essays Biochem. 1970;6:119-59 PMID: 5499232
  21. ACTIVATION AND INHIBITION OF DPN-LINKED ISOCITRATE DEHYDROGENASE OF HEART BY CERTAIN NUCLEOTIDES.
    Biochemistry. 1963 Sep-Oct;2:1023-32 PMID: 14087354
  22. Swelling and shrinkage phenomena in liver mitochondria. VI. Metabolism-independent swelling coupled to ion movement.
    Biochim Biophys Acta. 1967 May 9;131(3):468-78 PMID: 4962528
  23. [Production of alpha-hydroxyethyl-2-thiamine pyrophosphate with pyruvate oxidase from pig heart muscle].
    Biochem Z. 1961;334:473-86 PMID: 13749426
  24. Interconversion of phospho- and dephospho- forms of pig heart pyruvate dehydrogenase.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):947-54 PMID: 4314903
  25. Regulation of metabolism in working muscle in vivo. I. Concentrations of some glycolytic, tricarboxylic acid cycle, and amino acid intermediates in insect flight muscle during flight.
    J Biol Chem. 1966 Feb 10;241(3):624-31 PMID: 5908129
  26. Interaction of Ca2+ with blowfly flight muscle mitochondria.
    J Biol Chem. 1971 Feb 25;246(4):964-72 PMID: 5543696
  27. The swelling and contraction of isolated rat-liver mitochondria.
    Biochem J. 1964 Nov;93(2):322-30 PMID: 5838661
  28. The effect of the coupled oxidation of substrate on the permeability of blowfly flight-muscle mitochondria to potassium and other cations.
    Biochem J. 1972 Feb;126(3):689-700 PMID: 5075274
  29. Respiratory enzymes in oxidative phosphorylation. III. The steady state.
    J Biol Chem. 1955 Nov;217(1):409-27 PMID: 13271404
  30. Redistribution of the electrical charge of the mitochondrial membrane during energy conservation.
    Biochem Biophys Res Commun. 1969 Oct 8;37(2):254-60 PMID: 5823934
  31. On the role of K+ on oxidative phosphorylation.
    J Biol Chem. 1970 Oct 25;245(20):5239-47 PMID: 4319236
  32. The effects of Ca(2+) and ADP on the activity of NAD-linked isocitrate dehydrogenase of muscle.
    FEBS Lett. 1969 Oct 21;5(2):124-126 PMID: 11947257
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1270271
Published
1972-03-00
Pages
271-83
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178582
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1270271 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2