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PMID: 4343548 Published · ppublish English Journal Article

Deoxyribonucleic acid repair in bacteriophage T4: observations on the roles of the x and v genes and of host factors.

Journal of virology ·Vol. 10 ·No. 4 ·1972-10-00 ·Pages 730-6

Mortelmans K, Friedberg EC

Abstract

Studies were carried out to determine the effect of mutation in the host pol I gene on survival of ultraviolet (UV)-irradiated bacteriophage T4. Whereas a slightly reduced survival was observed in Escherichia coli strain P-3478 (pol A(1)) compared to strain W-3110 (pol A(+)), no such difference was observed in two strains isogenic except for the pol A gene. It was also shown that, whereas bacteriophage T4x is sensitive to UV irradiation, X irradiation, and treatment with methyl-methanesulfonate (MMS), phage T4v(1) is sensitive only to UV irradiation. The survival of damaged phage T4x is neither affected by the presence of the rec A, rec B, or pol A mutations in the host, nor is there evidence that phage T4 effects repair of rec A or pol A mutants previously treated with either UV or MMS.

MeSH Terms
Alkanesulfonates/pharmacology Cell Survival Cell-Free System Coliphages/drug effects,metabolism,radiation effects DNA Nucleotidyltransferases/metabolism DNA Repair DNA, Bacterial/biosynthesis DNA, Viral/biosynthesis Deoxyribonucleases/metabolism Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,metabolism,radiation effects Genes Mutation Radiation Effects Recombination, Genetic Ultraviolet Rays
Chemicals
Alkanesulfonates DNA, Bacterial DNA, Viral DNA Nucleotidyltransferases Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mortelmans K
Friedberg E C
References (30)
30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.4.730-736.1972
Published
1972-10-00
Pages
730-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356527
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.4.730-736.1972 PMC Full Text View journal details
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