Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3360-4

Keller W, Crouch R

Abstract

Ribonuclease H from human KB cells, chick embryos, calf thymus, avian myeloblastosis virus, and Rous associated virus specifically degrades the RNA of DNA.RNA hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. The cellular RNase H is an endonuclease, whereas the viral enzyme appears to be an exonuclease. Viral DNA polymerase and RNase H copurify through all separation steps. Therefore, RNase H activity is an intrinsic part of the viral DNA polymerase. DNA.RNA hybrids are also degraded by nucleases associated with cellular DNA polymerases and by exonuclease III. However, these nucleases differ from RNase H in their ability to degrade both strands of DNA.RNA hybrids.

MeSH Terms

Animals Avian Leukosis Virus/enzymology Avian Sarcoma Viruses/enzymology Cattle Cell Line Cells, Cultured Chick Embryo DNA/metabolism DNA Nucleotidyltransferases/metabolism Endonucleases/metabolism Escherichia coli/enzymology Exonucleases/metabolism Humans Nucleic Acid Hybridization Phosphorus Isotopes Polynucleotides/metabolism RNA/metabolism Ribonucleases/metabolism Ribonucleotides/biosynthesis Thymus Gland/enzymology

Chemicals

Phosphorus Isotopes Polynucleotides Ribonucleotides RNA DNA DNA Nucleotidyltransferases Endonucleases Exonucleases Ribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Keller W

Crouch R

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.11.3360

Published

1972-11-00

Pages

3360-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389771

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.11.3360 PMC Full Text View journal details

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Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.

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