Simplified radioimmunoassay for diagnostic serology.

Applied microbiology ·Vol. 24 ·No. 5 ·1972-11-00 ·Pages 742-9

Hutchinson HD, Ziegler DW

Abstract

A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with (125)I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the (125)I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.

MeSH Terms

Absorption Animals Antibodies, Bacterial/analysis Antibodies, Viral/analysis Antibody Formation Antigen-Antibody Reactions Antigens, Bacterial Antigens, Viral Complement Fixation Tests Escherichia coli/immunology Evaluation Studies as Topic Guinea Pigs/immunology Humans Immune Sera Immunization Immunologic Techniques Iodine Isotopes Rabbits/immunology Radioimmunoassay Serologic Tests Serum Globulins Simplexvirus/immunology Species Specificity Time Factors Vaccinia virus/immunology

Chemicals

Antibodies, Bacterial Antibodies, Viral Antigens, Bacterial Antigens, Viral Immune Sera Iodine Isotopes Serum Globulins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hutchinson H D

Ziegler D W

References (12)

12 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.24.5.742-749.1972

Published

1972-11-00

Pages

742-9

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380656

Subset

External Links

PubMed source DOI: 10.1128/am.24.5.742-749.1972 PMC Full Text

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