Spin-labeling studies on the membrane of a facultative thermophilic bacillus.

Journal of bacteriology ·Vol. 113 ·No. 1 ·1973-01-00 ·Pages 322-8

Chan M, Virmani YP, Himes RH, Akagi JM

Abstract

Spin-labeling studies were conducted to elucidate the rigidity and viscosity of the membrane of a facultative thermophilic bacillus. By employing nitroxide stearate probes, it was observed that this fatty acid was incorporated into the membrane fraction. Electron spin resonance spectroscopy indicated that cells grown at 55 C contained a membrane which was more rigid and viscous than the membrane from cells grown at 37 C.

MeSH Terms

Bacillus/analysis,metabolism Cell Membrane/analysis,metabolism Chromatography, Gas Chromatography, Thin Layer Electron Probe Microanalysis Electron Spin Resonance Spectroscopy Fatty Acids/analysis Fatty Acids, Nonesterified/analysis Hot Temperature Lipids/analysis Methods Nitrogen Oxides Phospholipids/analysis Spectrophotometry Stearic Acids/metabolism Viscosity

Chemicals

Fatty Acids Fatty Acids, Nonesterified Lipids Nitrogen Oxides Phospholipids Stearic Acids

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chan M

Virmani Y P

Himes R H

Akagi J M

References (10)

10 references, click to expand

Determination of fatty acid content and composition in ultramicro lipid samples by gas-liquid chromatography.
J Lipid Res. 1966 May;7(3):442-5 PMID: 5929359
Spin-labeled mitochondrial lipids in Neurospora crassa.
Proc Natl Acad Sci U S A. 1968 Nov;61(3):819-26 PMID: 4301148
Spin-label studies of the excitable membranes of nerve and muscle.
Proc Natl Acad Sci U S A. 1968 Sep;61(1):12-6 PMID: 4301585
Orientation and motion of amphiphilic spin labels in membranes.
Proc Natl Acad Sci U S A. 1969 Sep;64(1):20-7 PMID: 4312749
Physics and chemistry of spin labels.
Q Rev Biophys. 1970 Feb;3(1):91-136 PMID: 4908377
Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus.
J Bacteriol. 1970 Aug;103(2):479-81 PMID: 5432012
Spin-labeled Neurospora mitochondria.
Biophys J. 1970 Jul;10(7):618-29 PMID: 4317823
Motion of fatty acid spin labels in the plasma membrane of mycoplasma.
Biochim Biophys Acta. 1970;219(1):104-13 PMID: 4319690
Fatty acid composition of thermophilic, mesophilic, and psychrophilic clostridia.
J Bacteriol. 1971 Jun;106(3):876-81 PMID: 5567555
A simple method for the isolation and purification of total lipides from animal tissues.
J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.113.1.322-328.1973

Published

1973-01-00

Pages

322-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251634

Subset

External Links

PubMed source DOI: 10.1128/jb.113.1.322-328.1973 PMC Full Text View journal details

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Spin-labeling studies on the membrane of a facultative thermophilic bacillus.

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