Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells.

The Journal of cell biology ·Vol. 55 ·No. 1 ·1972-10-00 ·Pages 179-85

Plagemann PG, Estensen RD

Abstract

Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent K(i)'s of 2 and 6 microM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no direct effect on the synthesis of RNA, DNA, or uridine diphosphate-sugars. The inhibition of uridine and thymidine incorporation into nucleic acids by cytochalasin B is solely the consequence of the inhibition of nucleoside transport.

MeSH Terms

Animals Biological Transport/drug effects Carcinoma, Hepatocellular/metabolism Cells, Cultured Choline/metabolism DNA, Neoplasm/biosynthesis Indoles/pharmacology Liver Neoplasms/metabolism Neoplasms, Experimental/metabolism RNA, Neoplasm/biosynthesis Rats Thymidine/metabolism Tritium Uridine/metabolism Uridine Diphosphate Sugars/biosynthesis

Chemicals

DNA, Neoplasm Indoles RNA, Neoplasm Uridine Diphosphate Sugars Tritium Choline Thymidine Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Plagemann P G

Estensen R D

References (23)

23 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.55.1.179

Published

1972-10-00

Pages

179-85

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108743

Subset

External Links

PubMed source DOI: 10.1083/jcb.55.1.179 PMC Full Text View journal details

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Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells.

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