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PMID: 4347252 Published · ppublish English Journal Article

Effect of adenosine 3'-5'-cyclic monophosphate on cell proliferation.

The Journal of cell biology ·Vol. 55 ·No. 1 ·1972-10-00 ·Pages 19-31

Froehlich JE, Rachmeler M

Abstract

Secondary cultures of human diploid fibroblasts, which demonstrate density-dependent inhibition of cell growth, were used to study the effect of adenosine 3'-5'-cyclic monophosphate (cAMP) on cell proliferation. DNA synthesis in nonconfluent cultures and in contact-inhibited cultures stimulated to grow by refeeding with fresh medium was found to be inhibited by exogenous cAMP. The properties of this inhibition of DNA synthesis, together with the alterations in cAMP metabolism observed in confluent cultures of cells stimulated with fresh medium to resume growth, strongly suggest that cAMP is involved in contact-inhibition of cell proliferation.

MeSH Terms
Animals Biopsy Cell Division/drug effects Cells, Cultured Contact Inhibition Culture Media Cyclic AMP/analysis,metabolism,pharmacology DNA/biosynthesis Diploidy Fibroblasts Humans Immune Sera Kinetics Proteins/analysis Rabbits/immunology Radioimmunoassay Skin Theophylline/pharmacology Thymidine/metabolism Time Factors Tritium
Chemicals
Culture Media Immune Sera Proteins Tritium DNA Theophylline Cyclic AMP Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Froehlich J E
Rachmeler M
References (30)
30 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.55.1.19
Published
1972-10-00
Pages
19-31
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108750
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.55.1.19 PMC Full Text View journal details
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