Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1316-20

Orrick LR, Olson MO, Busch H

Abstract

A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.

MeSH Terms

Acetates Animals Buffers Carcinoma, Hepatocellular/metabolism Cell Nucleolus/analysis Electrophoresis, Polyacrylamide Gel Histones/isolation & purification Liver/analysis Liver Neoplasms/metabolism Methods Neoplasm Proteins/isolation & purification Neoplasms, Experimental Proteins/isolation & purification Rats Sodium Dodecyl Sulfate

Chemicals

Acetates Buffers Histones Neoplasm Proteins Proteins Sodium Dodecyl Sulfate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Orrick L R

Olson M O

Busch H

References (7)

7 references, click to expand

Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
Limited heterogeneity of the major nonhistone chromosomal proteins.
Biochemistry. 1970 Oct 27;9(22):4440-7 PMID: 5472713
Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.
J Biol Chem. 1971 Jun 10;246(11):3597-609 PMID: 4397103
Enumeration of rabbit reticulocyte ribosomal proteins.
J Mol Biol. 1971 Dec 14;62(2):403-5 PMID: 5138339
Isolation of an electrophoretically homogeneous nonhistone nucleolar protein.
Life Sci II. 1971 Nov 22;10(22):1297-309 PMID: 4335585
Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
J Biol Chem. 1972 Jul 25;247(14):4460-7 PMID: 5043849
Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate.
Cancer Res. 1972 Aug;32(8):1775-84 PMID: 5044136

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.5.1316

Published

1973-05-00

Pages

1316-20

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433487

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.5.1316 PMC Full Text View journal details

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Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

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