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PMID: 4351459 Published · ppublish English Comparative Study Journal Article

Radioimmunoassay for avian C-type virus group-specific antigen: detection in normal and virus-transformed cells.

Journal of virology ·Vol. 11 ·No. 6 ·1973-06-00 ·Pages 893-9

Stephenson JR, Wilsnack RE, Aaronson SA

Abstract

A radioimmunoassay has been developed for detection of avian C-type virus (30,000 mol wt) group-specific (gs) antigen. The method is 10- to 1,000-fold more sensitive than immunological methods previously available. By the radioimmunoassay technique, normal chicken embryo cells, which have previously been classified as gs negative or weakly gs positive, contain clearly detectable amounts of gs antigen. The assay has been used to study the effect of chemical induction and superinfection by mammalian C-type viruses on the expression of avian gs antigen in mammalian cells nonproductively transformed by avian sarcoma viruses.

MeSH Terms
Alpharetrovirus/immunology Animals Antigens, Viral/analysis Avian Sarcoma Viruses/growth & development Cell Line Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Complement Fixation Tests Electrophoresis, Polyacrylamide Gel Evaluation Studies as Topic Idoxuridine/pharmacology Iodine Isotopes Kidney Methods Mice Radioimmunoassay Rats Retroviridae/immunology Spectrophotometry
Chemicals
Antigens, Viral Iodine Isotopes Idoxuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stephenson J R
Wilsnack R E
Aaronson S A
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37 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.6.893-899.1973
Published
1973-06-00
Pages
893-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355197
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.6.893-899.1973 PMC Full Text View journal details
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