Home LiteratureArticle Details
PMID: 4351801 Published · ppublish English Journal Article

Nucleation, rapid folding, and globular intrachain regions in proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 3 ·1973-03-00 ·Pages 697-701

Wetlaufer DB

Abstract

Distinct structural regions have been found in several globular proteins composed of single polypeptide chains. The existence of such regions and the continuity of peptide chain within them, coupled with kinetic arguments, suggests that the early stages of three-dimensional structure formation (nucleation) occur independently in separate parts of these molecules. A nucleus can grow rapidly by adding peptide chain segments that are close to the nucleus in aminoacid sequence. Such a process would generate three-dimensional (native) protein structures that contain separate regions of continuous peptide chain. Possible means of testing this hypothesis are discussed.

MeSH Terms
Albumins Chymotrypsin Immunoglobulins L-Lactate Dehydrogenase Malate Dehydrogenase Models, Structural Muramidase Myoglobin Pancreatic Elastase Papain Phosphoglycerate Kinase Phosphoric Monoester Hydrolases Protein Conformation Ribonucleases Rubredoxins Serum Albumin, Bovine Subtilisins Thermolysin Trypsin Trypsin Inhibitors
Chemicals
Albumins Immunoglobulins Myoglobin Rubredoxins Trypsin Inhibitors Serum Albumin, Bovine L-Lactate Dehydrogenase Malate Dehydrogenase Phosphoglycerate Kinase Ribonucleases Phosphoric Monoester Hydrolases Muramidase Subtilisins Chymotrypsin Pancreatic Elastase Trypsin Papain Thermolysin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wetlaufer D B
References (32)
32 references, click to expand
  1. Structure of crystalline -chymotrypsin. V. The atomic structure of tosyl- -chymotrypsin at 2 A resolution.
    J Mol Biol. 1972 Jul 21;68(2):187-240 PMID: 5069789
  2. REVERSIBLE CONFORMATIONAL CHANGES OF MYOGLOBIN AND APOMYOGLOBIN.
    J Biol Chem. 1965 Jan;240:299-303 PMID: 14253427
  3. The crystal and molecular structure of DIP-inhibited bovine trypsin at2.7Angstrom resolution.
    Cold Spring Harb Symp Quant Biol. 1972;36:125-40 PMID: 4508129
  4. Structure of rubredoxin: an x-ray study to 2.5 A resolution.
    J Mol Biol. 1970 Jun 14;50(2):391-406 PMID: 5476919
  5. The quaternary structure of proteins.
    Angew Chem Int Ed Engl. 1966 Feb;5(2):231-45 PMID: 4956559
  6. Structure of horse-muscle phosphoglycerate kinase at 6 angstrom resolution.
    Nat New Biol. 1972 Feb 16;235(59):195-8 PMID: 4501531
  7. The stability of hydrogen-bonded peptide structures in aqueous solution.
    C R Trav Lab Carlsberg Chim. 1955;29(14-15):230-59 PMID: 13305131
  8. Folding of staphylococcal nuclease: kinetic studies of two processes in acid renaturation.
    J Mol Biol. 1971 Sep 28;60(3):499-508 PMID: 5110314
  9. Tertiary structure of ribonuclease.
    Nature. 1967 Mar 4;213(5079):862-5 PMID: 6043657
  10. Pancreatic trypsin inhibitor (Kunitz). I. Structure and function.
    Cold Spring Harb Symp Quant Biol. 1972;36:141-8 PMID: 4508131
  11. Structural studies and organic ligand-binding properties of bovine plasma albumin.
    J Biol Chem. 1970 Nov 25;245(22):6134-48 PMID: 5484470
  12. The three-dimensional structure of ribonuclease-S. Interpretation of an electron density map at a nominal resolution of 2 A.
    J Biol Chem. 1970 Jan 25;245(2):305-28 PMID: 5460889
  13. Crystal structure of an immunoglobulin molecule by x-ray diffraction and electron microscopy.
    Cold Spring Harb Symp Quant Biol. 1972;36:413-9 PMID: 4508154
  14. Subtilisin cleavage of bovine plasma albumin. Reversible association of the two primary fragments and their relation to the structure of the parent protein.
    Biochemistry. 1969 Jun;8(6):2357-65 PMID: 4979054
  15. A sequential model of nucleation-dependent protein folding: kinetic studies of ribonuclease A.
    J Mol Biol. 1972 Feb 14;63(3):453-69 PMID: 5014928
  16. Formation of three-dimensional structure in proteins. I. Rapid nonenzymic reactivation of reduced lysozyme.
    Biochemistry. 1970 Dec 8;9(25):5015-23 PMID: 4991411
  17. The structure of a calcium-binding protein from carp muscle.
    Cold Spring Harb Symp Quant Biol. 1972;36:217-20 PMID: 4508136
  18. Folding of polypeptide chains in proteins: a proposed mechanism for folding.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2293-7 PMID: 5289387
  19. Theoretical and experimental studies of conformations of polypeptides.
    Chem Rev. 1971 Apr;71(2):195-217 PMID: 4927987
  20. Synthesis of secretin. IV. Secondary structure in a miniature protein.
    J Am Chem Soc. 1969 Feb 12;91(4):944-9 PMID: 5778276
  21. Helix-coil transition of the isolated amino terminus of ribonuclease.
    Biochemistry. 1971 Feb 2;10(3):470-6 PMID: 5543977
  22. Some factors in the interpretation of protein denaturation.
    Adv Protein Chem. 1959;14:1-63 PMID: 14404936
  23. On the Structure of Native, Denatured, and Coagulated Proteins.
    Proc Natl Acad Sci U S A. 1936 Jul;22(7):439-47 PMID: 16577722
  24. Sensitive criteria for the critical size for helix formation in oligopeptides.
    Proc Natl Acad Sci U S A. 1969 Oct;64(2):444-50 PMID: 5261026
  25. Structural constraints of possible mechanisms of lactate dehydrogenase as shown by high resolution studies of the apoenzyme and a variety of enzyme complexes.
    Cold Spring Harb Symp Quant Biol. 1972;36:179-91 PMID: 4343715
  26. Structure and enzymatic break-down of proteins.
    Cold Spring Harb Symp Quant Biol. 1950;14:117-26 PMID: 15442905
  27. Conformational properties of cyanogen bromide-cleaved glucagon.
    J Biol Chem. 1972 Apr 10;247(7):2132-8 PMID: 5016646
  28. Reversible disruption by cupric ions of a helical conformation of a polypeptide derived from ribonuclease.
    J Biol Chem. 1966 Jun 10;241(11):2665-71 PMID: 5911639
  29. Some aspects of the structure of staphylococcal nuclease. I. Crystallographic studies.
    Cold Spring Harb Symp Quant Biol. 1972;36:243-9 PMID: 4343716
  30. The interpretation of protein structures: estimation of static accessibility.
    J Mol Biol. 1971 Feb 14;55(3):379-400 PMID: 5551392
  31. Tertiary structure in carboxypeptidase.
    J Am Chem Soc. 1972 Nov 29;94(24):8568-72 PMID: 4638988
  32. A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo.
    Cold Spring Harb Symp Quant Biol. 1972;36:107-16 PMID: 4508127
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.3.697
Published
1973-03-00
Pages
697-701
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433338
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.3.697 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2