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PMID: 4352178 Published · ppublish English Journal Article

Relationship of adenosine 3',5'-monophosphate to growth and metabolism of Tetrahymena pyriformis.

Journal of bacteriology ·Vol. 115 ·No. 1 ·1973-07-00 ·Pages 68-72

Voichick J, Elson C, Granner D, Shrago E

Abstract

The concentration of adenosine 3',5'-monophosphate (cyclic AMP) and the activity of adenylate cyclase were determined for the first time in conjuncation with cyclic 3',5'-nucleotide phosphodiesterase (phosphodiesterase) during the growth cycle of Tetrahymena pyriformis. High levels of cyclic AMP observed during early exponential and late stationary phases were associated with elevated adenylate cyclase and decreased phosphodiesterase activities. Adenylate cyclase and cyclic AMP were decreased and phosphodiesterase was increased in cells grown in glucose-supplemented medium. In contrast to findings in mammalian liver, cyclic AMP was decreased during active gluconeogenesis in Tetrahymena. This suggests a different modulation of carbohydrate metabolism in the two species. The results illustrate that both the content of cyclic AMP and its action as a regulatory agent in Tetrahymena are uniquely suited to the metabolism of this organism.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Carboxy-Lyases/metabolism Cell-Free System Chromatography Culture Media Cyclic AMP/metabolism Gluconeogenesis Glucose/metabolism Glycogen/biosynthesis Phosphoric Diester Hydrolases/metabolism Phosphorus Isotopes Tetrahymena pyriformis/enzymology,growth & development,metabolism Time Factors Tritium
Chemicals
Culture Media Phosphorus Isotopes Tritium Glycogen Cyclic AMP Phosphoric Diester Hydrolases Carboxy-Lyases Adenylyl Cyclases Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Voichick J
Elson C
Granner D
Shrago E
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.115.1.68-72.1973
Published
1973-07-00
Pages
68-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246213
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.115.1.68-72.1973 PMC Full Text View journal details
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