A mutant transcription factor that is activated by 3':5'-cyclic guanosine monophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1017-21

Sanders R, McGeoch D

Abstract

We report here the isolation of an extragenic suppressor of a promoter mutation in the lactose operon of Escherichia coli. Genetic data have indicated that the suppressor of the P(-) mutation maps in the structural gene for the transcription factor catabolite activator protein. Biochemical studies suggest that the mutant molecules of catabolite activator protein are altered in such a way that they are activated by 3':5'-cyclic GMP in addition to their normal effector, 3':5'-cyclic AMP.

MeSH Terms

Bacterial Proteins/biosynthesis,metabolism Chromosome Mapping Coliphages Cyclic GMP/pharmacology Escherichia coli/enzymology,metabolism Galactosidases/biosynthesis Genes Genetic Code Lactose/metabolism Mutation Operon Transcription, Genetic/drug effects Transduction, Genetic

Chemicals

Bacterial Proteins Galactosidases Cyclic GMP Lactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sanders R

McGeoch D

References (17)

17 references, click to expand

Mutagens which cause deletions in Escherichia coli.
Genetics. 1969 Feb;61(2):371-6 PMID: 4896998
Catabolite sensitive site of the lac operon.
Nature. 1969 Mar 15;221(5185):1012-4 PMID: 4886742
Current linkage map of Escherichia coli.
Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
Cyclic nucleotides.
Annu Rev Physiol. 1971;33:311-36 PMID: 4157117
Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
J Mol Biol. 1969 Aug 28;44(1):117-27 PMID: 4897798
Mutational alteration of the maximal level of Lac operon expression.
Cold Spring Harb Symp Quant Biol. 1966;31:403-8 PMID: 4866390
Genetic regulatory mechanisms in the synthesis of proteins.
J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
The nature of mutants in the lac promoter region.
J Mol Biol. 1968 Dec;38(3):421-6 PMID: 4887878
Fusions of the lac and trp Regions of the Escherichia coli Chromosome.
J Bacteriol. 1970 Dec;104(3):1273-9 PMID: 16559103
Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes.
Proc Natl Acad Sci U S A. 1970 Jun;66(2):480-7 PMID: 4317918
Mechanism of activation of catabolite-sensitive genes: a positive control system.
Proc Natl Acad Sci U S A. 1970 May;66(1):104-10 PMID: 4320461
Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32 PMID: 4331560
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
Virology. 1970 Mar;40(3):734-44 PMID: 4908735
Transduction of linked genetic characters of the host by bacteriophage P1.
Virology. 1955 Jul;1(2):190-206 PMID: 13267987
Transposition of the Lac region of E. coli.
Cold Spring Harb Symp Quant Biol. 1966;31:393-401 PMID: 4866389
A mechanism for repressor action.
J Mol Biol. 1969 Jul 14;43(1):201-13 PMID: 4897791
New controlling element in the Lac operon of E. coli.
Nature. 1968 Mar 2;217(5131):825-7 PMID: 4867974

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1017

Published

1973-04-00

Pages

1017-21

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433415

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.4.1017 PMC Full Text View journal details

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A mutant transcription factor that is activated by 3':5'-cyclic guanosine monophosphate.

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