Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1037-40

Sipe JD, de Maeyer-Guignard J, Fauconnier B, de Maeyer E

Abstract

A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.

MeSH Terms

Animals Cells, Cultured Chromatography Chromatography, Affinity Immune Sera Immunoglobulins Interferons/biosynthesis,isolation & purification Methods Mice Mice, Inbred C57BL Newcastle disease virus Parainfluenza Virus 1, Human Polysaccharides Sheep/immunology

Chemicals

Immune Sera Immunoglobulins Polysaccharides Interferons

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Sipe J D

de Maeyer-Guignard J

Fauconnier B

de Maeyer E

References (8)

8 references, click to expand

Chemical coupling of proteins to agarose.
Nature. 1967 Sep 30;215(5109):1491-2 PMID: 6059570
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
J Biol Chem. 1970 Jun;245(12):3059-65 PMID: 5432796
Antitumor effects of interferon preparations in mice.
J Natl Cancer Inst. 1970 Aug;45(2):365-76 PMID: 5514991
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Studies on virus-induced interferons produced by the human amniotic membrane and white blood cells.
Arch Gesamte Virusforsch. 1971;34(3):232-43 PMID: 4330138
Affinity chromatography.
Annu Rev Biochem. 1971;40:259-78 PMID: 4399260
Characterization of interferon-associated proteins.
J Gen Virol. 1972 May;15(2):129-38 PMID: 4338332
Effect of antilymphocytic serum on circulating interferon in mice as a function of the inducer.
Nat New Biol. 1971 Feb 17;229(7):212-4 PMID: 4325471

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1037

Published

1973-04-00

Pages

1037-40

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433420

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.4.1037 PMC Full Text View journal details

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Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.

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