Protein kinase activity in commercially available crystalline yeast alcohol dehydrogenase.

The Biochemical journal ·Vol. 131 ·No. 2 ·1973-02-00 ·Pages 271-4

Kemp BE, Froscio M, Murray AW

Abstract

Commercially available crystalline yeast alcohol dehydrogenase contained protein kinase activity. Casein and phosvitin were readily phosphorylated, but whole calf thymus histone was not. The protein kinase activity was inhibited by KCl, was not stimulated by cyclic AMP and could be separated from the alcohol dehydrogenase activity by sucrose density centrifugation.

MeSH Terms

Alcohol Oxidoreductases/analysis Animals Caseins Cattle Centrifugation, Density Gradient Chromatography, Gel Cyclic AMP Egg Proteins Evaluation Studies as Topic Histones Phosphoproteins Phosphorus Isotopes Phosphotransferases/analysis Potassium Chloride Protein Kinase Inhibitors Protein Kinases/analysis,isolation & purification,metabolism Saccharomyces cerevisiae/enzymology Spectrophotometry, Ultraviolet Thymus Gland

Chemicals

Caseins Egg Proteins Histones Phosphoproteins Phosphorus Isotopes Protein Kinase Inhibitors Potassium Chloride Cyclic AMP Alcohol Oxidoreductases Phosphotransferases Protein Kinases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kemp B E

Froscio M

Murray A W

References (11)

11 references, click to expand

Separation of a cyclic 3',5'-adenosine monophosphate binding protein from yeast.
Biochemistry. 1972 Jul 18;11(15):2784-7 PMID: 4339467
Multiple forms of hepatic adenosine 3':5'-monophosphate dependent protein kinase.
Biochemistry. 1971 Sep 14;10(19):3614-21 PMID: 4336415
Cyclic adenosine 3':5'-monophosphate and microtubule function: specific interaction of the phosphorylated protein subunits with a soluble brain component.
Biochem Biophys Res Commun. 1971 Sep;44(5):1089-95 PMID: 4334271
Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases.
J Biol Chem. 1971 Apr 10;246(7):1986-95 PMID: 4324558
Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom.
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349-55 PMID: 4393915
Reversible phosphate transfer between yolk phosphoprotein and adenosine triphosphate.
J Biol Chem. 1960 Apr;235:1043-50 PMID: 14435647
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
The role of zinc in alcohol dehydrogenase. V. The effect of metal-binding agents on thestructure of the yeast alcohol dehydrogenase molecule.
J Biol Chem. 1960 Nov;235:3188-92 PMID: 13750715
The grouping of serine phosphate residues in phosvitin and casein.
Biochim Biophys Acta. 1959 May;33(1):294-6 PMID: 13651231
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Alpha-keto acid dehydrogenase complexes. X. Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation.
Proc Natl Acad Sci U S A. 1969 Jan;62(1):234-41 PMID: 4306045

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310271

Published

1973-02-00

Pages

271-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177467

Subset

External Links

PubMed source DOI: 10.1042/bj1310271 PMC Full Text View journal details

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Protein kinase activity in commercially available crystalline yeast alcohol dehydrogenase.

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