Cyclic AMP and sodium transport. Quantitative and temporal relationships in toad urinary bladder.

The Journal of clinical investigation ·Vol. 52 ·No. 9 ·1973-09-00 ·Pages 2379-82

Sapirstein VS, Scott WN

Abstract

The effects of oxytocin upon tissue cAMP content and short-circuit current (SCC) were measured in the urinary bladder of the toad, Bufo marinus. Tissue cAMP levels doubled before any increment in SCC was observed, the two hormone responses were quantitatively related, and a threshold level for an effect of cAMP upon sodium transport was demonstrated. The period of time over which cAMP levels continued to rise after the threshold level had been attained seemed invariant with hormone concentration. The rate at which cAMP levels rose increased with hormone concentration yielding hormone concentration-dependent maximal levels. The decay of cAMP levels was delayed when sodium influx was curtailed, suggesting a sodium-regulatory effect upon tissue cAMP levels.

MeSH Terms

Animals Biological Transport Bufo marinus Culture Media Cyclic AMP/analysis,metabolism,physiology In Vitro Techniques Oxytocin/pharmacology Sodium/metabolism Stimulation, Chemical Urinary Bladder/analysis,drug effects,metabolism

Chemicals

Culture Media Oxytocin Sodium Cyclic AMP

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sapirstein V S

Scott W N

References (11)

11 references, click to expand

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Proc Natl Acad Sci U S A. 1969 Sep;64(1):367-73 PMID: 4189501
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J Biol Chem. 1967 Dec 10;242(23):5535-41 PMID: 12325369
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Active sodium transport by the isolated toad bladder.
J Gen Physiol. 1958 Mar 20;41(4):657-68 PMID: 13514002
Threshold and receptor reserve in the action of neurohypophyseal peptides. A study of synergists and antagonists of the hydroosmotic response of the toad urinary bladder.
J Gen Physiol. 1970 Aug;56(2):250-71 PMID: 5433469
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Proc Natl Acad Sci U S A. 1970 Sep;67(1):7-12 PMID: 5272332
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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI107426

Published

1973-09-00

Pages

2379-82

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC333042

Subset

External Links

PubMed source DOI: 10.1172/JCI107426 PMC Full Text View journal details

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Cyclic AMP and sodium transport. Quantitative and temporal relationships in toad urinary bladder.

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