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PMID: 4355929 Published · ppublish English Journal Article

Hydroxyapatite chromatography and formamide denaturation of adenovirus DNA.

Journal of virology ·Vol. 12 ·No. 2 ·1973-08-00 ·Pages 218-25

Tibbetts C, Johansson K, Philipson L

Abstract

Denatured adenovirus DNA was retained by hydroxyapatite columns under conditions generally used for selective retention of double-stranded DNA, probably due to several partially complementary sequences within single-stranded DNA. It was found that addition of formamide reduced the fraction of sonically treated, denatured adenovirus DNA bound to hydroxyapatite from about 30% to less than 1%. This led to a study of the effect of formamide on the melting temperature (T(m)) of double-stranded DNA in solution or bound to hydroxyapatite. The T(m) of DNA decreases 0.56 C/1% formamide, a value determined in buffered solutions with purified formamide.

MeSH Terms
Adenoviridae/analysis Carbon Radioisotopes Centrifugation, Density Gradient Chromatography/standards DNA, Single-Stranded/analysis,isolation & purification DNA, Viral/analysis,isolation & purification Formamides Hot Temperature Hydrogen-Ion Concentration Hydroxyapatites Methods Molecular Weight Nucleic Acid Denaturation Nucleic Acid Renaturation Simian virus 40/analysis Spectrophotometry Tritium Vibration
Chemicals
Carbon Radioisotopes DNA, Single-Stranded DNA, Viral Formamides Hydroxyapatites Tritium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tibbetts C
Johansson K
Philipson L
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21 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.2.218-225.1973
Published
1973-08-00
Pages
218-25
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356615
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.2.218-225.1973 PMC Full Text View journal details
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