An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth.

The Biochemical journal ·Vol. 134 ·No. 4 ·1973-08-00 ·Pages 1051-61

Downie JA, Garland PB

Abstract

1. During copper-limited growth of Candida utilis in continuous culture on a non-fermentable carbon and energy source there is a selective pressure favouring the emergence of variants that are less dependent on copper. 2. We describe the properties of such a variant that by-passes cytochrome oxidase (EC 1.9.3.1) by utilizing an alternative oxidase communicating with the respiratory chain at about the level of cytochrome b. 3. Both direct studies of isolated mitochondria and calculations based on growth parameters showed that only one of the normal three phosphorylation sites was active. This site was localized between NADH and the cytochromes. 4. Growth of the variant with copper-supplemented media resulted in the return of cytochrome oxidase but not the loss of the alternative oxidase. 5. The alternative oxidase is inhibited by substituted benzhydroxamic acids. 6. Submitochondrial particles from the variant did not exhibit any novel electron-paramagnetic-resonance-spectroscopy features at about g=2.0 either at 80 degrees K or 12 degrees K.

MeSH Terms

Antimycin A/pharmacology Binding Sites Candida/growth & development,metabolism Copper/metabolism Culture Media Cyanides/pharmacology Drug Resistance, Microbial Electron Spin Resonance Spectroscopy Electron Transport Complex IV Mitochondria/metabolism Oxidation-Reduction

Chemicals

Culture Media Cyanides Antimycin A Copper Electron Transport Complex IV

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Downie J A

Garland P B

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1341051

Published

1973-08-00

Pages

1051-61

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177914

Subset

External Links

PubMed source DOI: 10.1042/bj1341051 PMC Full Text View journal details

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An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth.

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