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PMID: 4357874 Published · ppublish English Journal Article

Requirement of a phage-induced 5'-exonuclease for the expression of late genes of bacteriophage T5.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3502-5

Chinnadurai G, McCorquodale DJ

Abstract

Amber mutants of bacteriophage T5 defective in gene D15, which codes for a 5'-exonuclease, do not express late genes. Electrophoretic separation in sodium dodecyl sulfate-polyacrylamide gels of the proteins induced by this mutant in nonpermissive Escherichia coli show a virtual absence of late proteins. Synthesis of lysozyme and serum-blocking power is very low whereas the extent of synthesis of an early enzyme, deoxyribonucleoside monophosphokinase, is similar to that in wild-type infections. It is proposed that one requirement for the expression of late T5 genes is the introduction of gaps or nicks in the T5 DNA so that late transcription can occur.

MeSH Terms
Carbon Radioisotopes Coliphages/enzymology,metabolism Deoxyribonucleotides Electrophoresis, Polyacrylamide Gel Escherichia coli Exonucleases/biosynthesis,metabolism Kinetics Leucine/metabolism Lysogeny Muramidase/biosynthesis Mutation Phosphotransferases/biosynthesis Time Factors Viral Proteins/analysis,biosynthesis
Chemicals
Carbon Radioisotopes Deoxyribonucleotides Viral Proteins Phosphotransferases Exonucleases Muramidase Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chinnadurai G
McCorquodale D J
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.12.3502
Published
1973-12-00
Pages
3502-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427268
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.12.3502 PMC Full Text View journal details
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