Home LiteratureArticle Details
PMID: 4359488 Published · ppublish English Journal Article

Size and base composition of RNA in supercoiled plasmid DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3744-8

Williams H, Boyer HW, Helsinki DR

Abstract

The average size and base composition of the covalently integrated RNA segment in supercoiled ColE(1) DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE(1) DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE(1) DNA contains GMP at the 5' end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.

MeSH Terms
Adenosine Monophosphate/metabolism Alkalies/metabolism Avian Sarcoma Viruses/enzymology Base Sequence Chloramphenicol/pharmacology Cytosine Nucleotides/metabolism DNA Nucleotidyltransferases/metabolism DNA, Bacterial/analysis,biosynthesis,metabolism Electrophoresis, Paper Escherichia coli/analysis,cytology,enzymology Guanine Nucleotides/metabolism Inclusion Bodies/analysis Nucleotides/analysis Phosphorus Radioisotopes Polynucleotide Ligases/metabolism RNA, Bacterial/analysis Ribonucleases/metabolism Ribonucleotides/analysis Thymine Nucleotides/metabolism
Chemicals
Alkalies Cytosine Nucleotides DNA, Bacterial Guanine Nucleotides Nucleotides Phosphorus Radioisotopes RNA, Bacterial Ribonucleotides Thymine Nucleotides Adenosine Monophosphate Chloramphenicol DNA Nucleotidyltransferases Ribonucleases Polynucleotide Ligases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams H
Boyer H W
Helsinki D R
References (28)
28 references, click to expand
  1. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  2. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  3. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.
    J Mol Biol. 1968 Sep 14;36(2):185-94 PMID: 4939624
  4. A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9 PMID: 4941987
  5. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T.
    J Mol Biol. 1972 Feb 28;64(1):47-60 PMID: 4552485
  6. Inhibition of plasmid DNA replication by rifampin in Salmonella pullorum.
    Biochem Biophys Res Commun. 1972 Mar 24;46(6):2019-25 PMID: 5018666
  7. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 PMID: 4554537
  8. Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus.
    Biochemistry. 1972 Jun 6;11(12):2334-42 PMID: 4337614
  9. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin.
    Nat New Biol. 1972 May 3;237(70):29-31 PMID: 4337979
  10. Replication of bacteriophage M 13: inhibition of single-strand DNA synthesis by rifampicin.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1634-7 PMID: 4556466
  11. RNA-linked nascent DNA fragments in Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863-7 PMID: 4558661
  12. Inhibition of minicircular DNA replication in Escherichia coli 15 by rifampicin.
    Nat New Biol. 1972 Aug 16;238(85):202-3 PMID: 4559665
  13. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518-22 PMID: 4560690
  14. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 PMID: 4560696
  15. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation.
    J Bacteriol. 1972 Oct;112(1):7-12 PMID: 4562418
  16. In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3165-9 PMID: 4564205
  17. DNA nucleotide sequence restricted by the RI endonuclease.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448-52 PMID: 4343974
  18. Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.
    J Virol. 1972 Nov;10(5):902-8 PMID: 4564585
  19. RNA bound to nascent DNA in Ehrlich ascites tumor cells.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):827-34 PMID: 4674116
  20. Assymetric annealing of an RNA linked DNA molecule isolated during the initiation of bacteriophage T7 DNA replication.
    Biochem Biophys Res Commun. 1972 Nov 15;49(4):1082-6 PMID: 4565800
  21. RNA-linked DNA fragments in vitro.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):88-92 PMID: 4567338
  22. Initiation of deoxyribonucleic acid synthesis. IV. Incorporation of the ribonucleic acid primer into the phage replicative form.
    J Biol Chem. 1973 Feb 25;248(4):1361-4 PMID: 4568814
  23. RNase and alkali sensitivity of closed circular mitochondrial DNA of rat ascites hepatoma cells.
    Biochem Biophys Res Commun. 1973 Jan 23;50(2):252-8 PMID: 4347514
  24. Role of DNA transcription in the initiation of Escherichia coli sex factor (F) DNA replication.
    Biochem Biophys Res Commun. 1973 Jan 23;50(2):280-8 PMID: 4569874
  25. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  26. DNA substrate site for the EcoRII restriction endonuclease and modification methylase.
    Nat New Biol. 1973 Jul 11;244(132):40-3 PMID: 4124385
  27. Effects of rifampicin, streptolydigin and actinomycin D on the replication of Col E1 plasmid DNA in Escherichia coli.
    J Mol Biol. 1973 Apr 15;75(3):503-13 PMID: 4579715
  28. Nucleotide sequence determination of bacteriophage T4 leucine transfer ribonucleic acid.
    J Biol Chem. 1973 Sep 25;248(18):6348-65 PMID: 4354208
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.12.3744
Published
1973-12-00
Pages
3744-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427319
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.12.3744 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2