Home LiteratureArticle Details
PMID: 4359950 Published · ppublish English Journal Article

Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus.

Journal of virology ·Vol. 12 ·No. 4 ·1973-10-00 ·Pages 721-32

Markham PD, Baluda MA

Abstract

The covalent linkage of oncornavirus-specific DNA to chicken DNA was investigated in normal chicken embryo fibroblasts (CEF) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (AMV). The virus-specific sequences present in cellular DNA fractionated by different methods were detected by DNA-RNA hybridization by using 70S AMV RNA as a probe. In CEF and in leukemic cells, the viral DNA appeared to be present only in the nucleus. After cesium chloride-ethidium bromide density equilibrium sedimentation, the viral DNA was present as linear, double-stranded molecules not separable from linear chicken DNA. After extraction by the Hirt procedure, the viral DNA precipitated with the high-molecular-weight DNA. After alkaline sucrose velocity sedimentation, the viral DNA cosedimented with the high-molecular-weight cellular DNA. The results indicate that in both types of cells studied, the oncornavirus-specific DNA sequences were linked by alkali stable bonds to nuclear cellular DNA of high molecular weight and did not appear to be present in free form of any size.

MeSH Terms
Animals Avian Leukosis Virus Carbon Radioisotopes Cell Fractionation Cell Nucleus/analysis Cell Transformation, Neoplastic Cells, Cultured Centrifugation, Density Gradient Chick Embryo DNA/analysis,isolation & purification DNA, Mitochondrial/isolation & purification DNA, Viral/metabolism Fibroblasts Isotope Labeling Nucleic Acid Hybridization Oncogenic Viruses Proteins/analysis RNA/analysis RNA, Viral/classification Tritium
Chemicals
Carbon Radioisotopes DNA, Mitochondrial DNA, Viral Proteins RNA, Viral Tritium RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Markham P D
Baluda M A
References (47)
47 references, click to expand
  1. Mitochondrial DNA. II. Structure and physicochemical properties of isolated DNA.
    J Mol Biol. 1969 Jun 28;42(3):529-45 PMID: 5816966
  2. Mitochondrial DNA: Advances, Problems, and Goals.
    Science. 1969 Jul 4;165(3888):25-35 PMID: 17840675
  3. Genetic transmission of viruses that incite mammary tumor in mice.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):377-84 PMID: 4318784
  4. RNA-directed DNA synthesis and RNA tumor viruses.
    Adv Virus Res. 1972;17:129-86 PMID: 4348509
  5. Mitochondrial DNA. I. Intramitochondrial distribution and structural relations of single- and double-length circular DNA.
    J Mol Biol. 1969 Jun 28;42(3):521-8 PMID: 5816965
  6. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  7. Induction of virus synthesis in polyoma-transformed cells by DNA antimetabolites and by irradiation after pretreatment with 5-bromodeoxyuridine.
    Virology. 1972 Jul;49(1):12-22 PMID: 4338990
  8. Recovery of the temperature-sensitive mutant of Rous sarcoma virus from chicken cells exposed to DNA extracted from hamster cells transformed by the mutant.
    Virology. 1972 Jul;49(1):309-13 PMID: 4338994
  9. Detection of avian tumor virus-specific nucleotide sequences in avian cell DNAs (reassociation kinetics-RNA tumor viruses-gas antigen-Rous sarcoma virus, chick cells).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):20-4 PMID: 4333039
  10. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.
    Nature. 1973 Mar 2;242(5392):44-7 PMID: 4348458
  11. Separation of Epstein-Barr virus DNA from large chromosomal DNA in non-virus-producing cells.
    Nat New Biol. 1972 Aug 9;238(84):169-71 PMID: 4340572
  12. Studies on the nature and genetic control of an antigen in normal chick embryos which reacts in the COFAL test.
    J Gen Virol. 1968 Dec;3(3):379-91 PMID: 4304102
  13. An avian leukosis virus related to RSV(O): properties and evidence for helper activity.
    Virology. 1971 Jan;43(1):223-34 PMID: 5101017
  14. DNA ligase and exonuclease activities in virions of rous sarcoma virus.
    Nat New Biol. 1971 Apr 21;230(16):232-5 PMID: 4324864
  15. Lysogenic induction.
    Prog Nucleic Acid Res Mol Biol. 1973;13:249-300 PMID: 4267058
  16. Acquisition of sequences homologous to host deoxyribonucleic acid by closed circular simian virus 40 deoxyribonucleic acid.
    J Virol. 1972 Feb;9(2):309-16 PMID: 4335520
  17. The integrated state of viral DNA in SV40-transformed cells.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288-95 PMID: 4299943
  18. A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784-9 PMID: 4345508
  19. Virus particles and viral antigens in chicken tissues free of infectious avian leukosis virus.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):808-17 PMID: 5233847
  20. Morphological conversion of cell cultures by avian myeloblastosis virus.
    Virology. 1961 Oct;15:185-99 PMID: 13864532
  21. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336-40 PMID: 4332808
  22. Rescue of rous sarcoma virus from rous sarcoma virus-transformed mammalian cells.
    J Virol. 1972 Jul;10(1):153-6 PMID: 4339192
  23. Recent advances in molecular pathology. The state of the viral genome in cells transformed by simian virus 40: a review.
    Exp Mol Pathol. 1972 Aug;17(1):103-19 PMID: 4339647
  24. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  25. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21 PMID: 5231757
  26. Three size-classes of intracellular adenovirus deoxyribonucleic acid.
    J Virol. 1971 Jun;7(6):707-19 PMID: 5105670
  27. Temperature-dependent transformation of cells infected with a mutant of Bryan Rous sarcoma virus.
    J Virol. 1972 Aug;10(2):267-76 PMID: 4342244
  28. Oncogenic viruses.
    Annu Rev Biochem. 1970;39:701-56 PMID: 4320675
  29. Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of permissive monkey kidney cells.
    J Virol. 1972 Apr;9(4):705-7 PMID: 4336565
  30. Prophage P1, and extrachromosomal replication unit.
    Cold Spring Harb Symp Quant Biol. 1968;33:791-8 PMID: 4892009
  31. Distribution of deoxyribonucleic acid complementary to the ribonucleic acid of avian myeloblastosis virus in tissues of normal and tumor-bearing chickens.
    J Virol. 1972 Nov;10(5):1002-9 PMID: 4344249
  32. DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus.
    Proc Natl Acad Sci U S A. 1970 Jun;66(2):329-36 PMID: 4317913
  33. 'Integration' of polyoma virus DNA into mammalian genomes.
    Biochem Biophys Res Commun. 1972 Apr 14;47(1):111-8 PMID: 4337421
  34. Lysogeny: viral repression and site-specific recombination.
    Annu Rev Biochem. 1971;40:827-54 PMID: 4941241
  35. Carcinogenesis by avian sarcoma viruses.
    Cancer Res. 1968 Sep;28(9):1835-8 PMID: 4300222
  36. Rous sarcoma virus nucleotide sequences in cellular DNA: measurement by RNA-DNA hybridization.
    Science. 1972 Nov 17;178(4062):750-3 PMID: 4343259
  37. Detection of avian tumor virus RNA in uninfected chicken embryo cells.
    J Virol. 1973 Feb;11(2):157-67 PMID: 4120205
  38. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80 PMID: 4335067
  39. Rous sarcoma virus: a function required for the maintenance of the transformed state.
    Nature. 1970 Sep 5;227(5262):1021-3 PMID: 4317808
  40. Possible implications for medicine of RNA-directed DNA synthesis.
    Triangle. 1972;11(2):37-42 PMID: 4347271
  41. The nucleic acid from avian myeloblastosis virus compared with the RNA from the Bryan strain of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1965 Dec;54(6):1686-92 PMID: 4286563
  42. Mechanism of cell transformation by RNA tumor viruses.
    Annu Rev Microbiol. 1971;25:609-48 PMID: 4342705
  43. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions.
    J Virol. 1969 Nov;4(5):554-66 PMID: 4311791
  44. Isolation of leukosis-type virus from pheasant embryo cells: possible presence of viral genes in cells.
    Virology. 1973 Jan;51(1):247-51 PMID: 4346298
  45. Some implications of the activation of murine leukemia virus by halogenated pyrimidines.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):1033-5 PMID: 4337234
  46. Nucleotide composition of RNA hybridized to homologous DNA from cells transformed by avian tumour viruses.
    Nat New Biol. 1971 May 19;231(20):90-1 PMID: 4325656
  47. The heritable nature of the factor in chicken cells which acts as a helper virus for Rous sarcoma virus.
    Virology. 1971 Aug;45(2):508-15 PMID: 4328812
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.12.4.721-732.1973
Published
1973-10-00
Pages
721-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356690
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.12.4.721-732.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2