Home LiteratureArticle Details
PMID: 4360949 Published · ppublish English Journal Article

31P nuclear magnetic resonance spectroscopy of native and recombined lipoproteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 2 ·1974-02-00 ·Pages 549-53

Assmann G, Sokoloski EA, Brewer HB

Abstract

Native and recombined lipoproteins have been studied by (31)P nuclear magnetic resonance spectroscopy. Very low-, low-, and high-density lipoproteins exhibited characteristic spectra. The main resonances were assigned to phosphatidylcholine and sphingomyelin. Relaxation times for these phospholipids were separately measured in low-density lipoproteins and high-density lipoproteins. The effect of paramagnetic ions (Eu(+++)) on the nuclear magnetic resonance spectrum of high-density lipoproteins is reported.

MeSH Terms
Europium Humans Lipoproteins Lipoproteins, HDL Lipoproteins, LDL Lipoproteins, VLDL Magnetic Resonance Spectroscopy Phosphatidylcholines Phosphatidylethanolamines Phosphatidylinositols Phosphorus Isotopes Sphingomyelins
Chemicals
Lipoproteins Lipoproteins, HDL Lipoproteins, LDL Lipoproteins, VLDL Phosphatidylcholines Phosphatidylethanolamines Phosphatidylinositols Phosphorus Isotopes Sphingomyelins Europium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Assmann G
Sokoloski E A
Brewer H B
References (29)
29 references, click to expand
  1. Improved reagent for trimethylsilylation of sphingolipid bases.
    J Lipid Res. 1967 Jul;8(4):391-5 PMID: 6033606
  2. Studies on the conformation of human serum high-density lipoproteins HDL2 and HDL3.
    Proc Natl Acad Sci U S A. 1965 Dec;54(6):1699-705 PMID: 5218921
  3. Structure of human serum lipoproteins: nuclear magnetic resonance supports a micellar model.
    Science. 1968 Nov 22;162(3856):909-11 PMID: 5684497
  4. 220 MHz nuclear magnetic resonance spectra of high density serum lipoproteins.
    Nature. 1969 Jan 18;221(5177):260-1 PMID: 5763080
  5. The structure of human density lipoprotein: a study of the effect of phospholipase A and trypsin on its components and of the behavior of the lipid and protein moieties at the air-water interphase.
    Biochim Biophys Acta. 1969 Mar;175(2):290-300 PMID: 5778170
  6. Nuclear magnetic resonance studies on serum low density lipoproteins (LDL2).
    Chem Phys Lipids. 1969 Apr;3(2):152-8 PMID: 5784125
  7. Lipid abnormalities in foam cell reticulosis of mice, an analogue of human sphingomyelin lipidosis.
    J Lipid Res. 1969 May;10(3):288-93 PMID: 4306614
  8. High-resolution NMR spectra of high-density serum lipoproteins.
    Biochim Biophys Acta. 1969 Apr 29;176(3):524-36 PMID: 5800042
  9. Conformation of human serum high density lipoprotein and its peptide components.
    Nature. 1969 Oct 4;224(5214):69-70 PMID: 5822907
  10. Application of electron spin resonance to the study of the structure of human serum lipoproteins.
    Biochem Biophys Res Commun. 1969 Oct 22;37(3):444-50 PMID: 4310668
  11. Isolation and characterization of polypeptides of human serum lipoproteins.
    Biochemistry. 1969 Nov;8(11):4510-6 PMID: 5353112
  12. Electron spin resonance studies of lipid-protein interaction in human serum lipoproteins.
    Proc Natl Acad Sci U S A. 1970 Jan;65(1):145-51 PMID: 4313193
  13. Recent studies on the structure of human serum low-and high-density lipoproteins.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1119-27 PMID: 5264142
  14. The effect of reduction and carboxymethylation on the circular dichroic spectra of two polypeptide classes of serum high density lipoprotein.
    Biochim Biophys Acta. 1970 Mar 31;200(3):570-2 PMID: 5436648
  15. Observations on the conformation of human serum high-density lipoproteins using infrared spectroscopy, circular dichroism, and electron spin resonance.
    Biochemistry. 1970 Oct 27;9(22):4276-83 PMID: 4319599
  16. Fluorescence studies of a high density serum lipoprotein.
    Chem Phys Lipids. 1970 Aug;4(3):367-74 PMID: 5507522
  17. Mass spectral analysis glycerophospholipids.
    Biochemistry. 1971 Mar 16;10(6):927-32 PMID: 4323852
  18. A simple chemical method for labelling phosphatidylcholine and sphingomyelin in the choline moiety.
    Hoppe Seylers Z Physiol Chem. 1971 Aug;352(8):1058-64 PMID: 5098348
  19. On the metabolism of sphinganyl- and sphingenyl-1-phosphorylcholine.
    Hoppe Seylers Z Physiol Chem. 1972 Jan;353(1):65-74 PMID: 4335782
  20. Isolation and characterization of apoLp-Gln-II (apoA-II), a plasma high density apolipoprotein containing two identical polypeptide chains.
    J Biol Chem. 1972 Dec 10;247(23):7510-8 PMID: 4344224
  21. Small-angle x-ray scattering of human serum high-density lipoproteins.
    J Supramol Struct. 1972;1(2):98-104 PMID: 4347292
  22. Natural abundance carbon-13 nuclear magnetic resonance spectra of human serum lipoproteins.
    Science. 1973 Apr 15;180(4082):193-5 PMID: 4348464
  23. Transbilayer asymmetry and surface homogeneity of mixed phospholipids in cosonicated vesicles.
    Biochemistry. 1973 Jul 3;12(14):2637-45 PMID: 4736410
  24. Lanthanide ion-induced isotropic shifts and broadening for nuclear magnetic resonance structural analysis of model membranes.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1814-8 PMID: 4515942
  25. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  26. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  27. THE ACTION OF ENZYMES OF HUMAN ALPHA-LIPOPROTEIN.
    Biochem J. 1963 Dec;89:561-4 PMID: 14101977
  28. METHODS FOR METHANOLYSIS OF SPHINGOLIPIDS AND DIRECT DETERMINATION OF LONG-CHAIN BASES BY GAS CHROMATOGRAPHY.
    J Am Oil Chem Soc. 1965 Apr;42:294-8 PMID: 14279115
  29. On the structure of human serum high-density lipoprotein: studies by the technique of circular dichroism.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):890-4 PMID: 5238669
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.2.549
Published
1974-02-00
Pages
549-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388045
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.2.549 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2