Home LiteratureArticle Details
PMID: 4362626 Published · ppublish English Journal Article

In vitro activation of the transcription of araBAD operon by araC activator.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 634-8

Lee N, Wilcox G, Gielow W, Arnold J, Cleary P, Englesberg E

Abstract

The transcription of araBAD operon requires araC activator and cyclic AMP. D-Fucose inhibits ara mRNA synthesis. Our results indicate that the positive control by araC activator is exerted at the level of transcription.

MeSH Terms
Arabinose/metabolism Bacterial Proteins/metabolism Chromosome Mapping Coliphages Cyclic AMP/pharmacology DNA, Viral Escherichia coli/metabolism Fucose/pharmacology Lysogeny Nucleic Acid Hybridization Operon RNA, Bacterial/analysis,biosynthesis RNA, Messenger/analysis,biosynthesis Templates, Genetic Transcription, Genetic/drug effects Transduction, Genetic Tritium
Chemicals
Bacterial Proteins DNA, Viral RNA, Bacterial RNA, Messenger Tritium Fucose Arabinose Cyclic AMP
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee N
Wilcox G
Gielow W
Arnold J
Cleary P
Englesberg E
References (17)
17 references, click to expand
  1. The ribonuclease activity of crystallized pancreatic deoxyribonuclease.
    Anal Biochem. 1966 Feb;14(2):269-77 PMID: 5939865
  2. Further evidence for positive control of the L-arabinose system by gene araC.
    J Mol Biol. 1967 May 14;25(3):443-54 PMID: 5340694
  3. Initiation and patterns of transcription during phage development.
    Proc Can Cancer Conf. 1969;8:183-215 PMID: 4893426
  4. Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing bacteriophages for any Escherichia coli gene.
    J Mol Biol. 1969 Aug 28;44(1):117-27 PMID: 4897798
  5. Polarity and the degradation of mRNA.
    Nature. 1969 Oct 25;224(5217):329-31 PMID: 4898925
  6. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6160-7 PMID: 4900510
  7. Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.
    J Bacteriol. 1970 May;102(2):593-5 PMID: 4911550
  8. Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions.
    J Mol Biol. 1970 Aug;51(3):541-50 PMID: 4923859
  9. Reversible denaturation of enzymes by sodium dodecyl sulfate.
    J Biol Chem. 1971 Jul 25;246(14):4504-9 PMID: 5106387
  10. On the mechanism of action of lac repressor.
    Nat New Biol. 1971 Sep 15;233(37):67-70 PMID: 4330295
  11. Arabinose C protein: regulation of the arabinose operon in vitro.
    Nat New Biol. 1971 Oct 6;233(40):166-70 PMID: 4330551
  12. Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.
    J Bacteriol. 1971 Oct;108(1):1-4 PMID: 4941555
  13. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens.
    J Mol Biol. 1972 Feb 14;63(3):483-503 PMID: 4552408
  14. In vitro repression of the transcription of gas operon by purified gal repressor.
    Proc Natl Acad Sci U S A. 1973 Feb;70(2):334-8 PMID: 4346883
  15. Regulatory gene control of transcription of the L-arabinose operon in Escherichia coli.
    J Biol Chem. 1973 Nov 25;248(22):7806-10 PMID: 4584341
  16. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  17. THE OPERON: ON ITS THIRD ANNIVERSARY. MODULATION OF TRANSFER RNA SPECIES CAN PROVIDE A WORKABLE MODEL OF AN OPERATOR-LESS OPERON.
    Science. 1964 May 15;144(3620):816-20 PMID: 14149392
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.3.634
Published
1974-03-00
Pages
634-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388066
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.3.634 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2