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PMID: 4366245 Published · ppublish English Journal Article

Effects of phorbol myristate acetate on the metabolism and ultrastructure of neutrophils in chronic granulomatous disease.

The Journal of clinical investigation ·Vol. 54 ·No. 1 ·1974-07-00 ·Pages 83-90

Repine JE, White JG, Clawson CC, Holmes BM

Abstract

Previous investigations have demonstrated that phorbol myristate acetate (PMA), the active principle of croton oil, stimulates alterations in normal polymorphonuclear leukocytes (PMN) that resemble closely the changes that develop in the cells after phagocytosis of bacteria. The present study has compared the effects of PMA and heat-killed bacteria on the oxygen uptake, glucose oxidation, nitroblue tetrazolium (NBT) reduction, and ultrastructure of normal neutrophils and PMN from six patients with chronic granulomatous disease (CGD). PMA stimulated oxygen consumption, hexose monophosphate shunt activity, and NBT reduction in normal cells but failed to produce similar effects in CGD neutrophils. However, PMA did induce formation of cytoplasmic vacuoles in the CGD cells similar to those observed in normal neutrophils. The results indicate that PMA is a useful nonparticulate agent for distinguishing between normal and CGD neutrophils and for studying basic mechanisms of phagocytosis in normal and abnormal PMN.

MeSH Terms
Alcohols/pharmacology Croton Oil/pharmacology Diterpenes/pharmacology Fatty Acids/pharmacology Female Glucose/metabolism Humans Inclusion Bodies Male Microscopy, Electron Neutrophils/cytology,drug effects,metabolism Oxygen Consumption Phagocyte Bactericidal Dysfunction/blood,metabolism,pathology Phagocytosis Tetrazolium Salts/metabolism
Chemicals
Alcohols Diterpenes Fatty Acids Tetrazolium Salts Croton Oil Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Repine J E
White J G
Clawson C C
Holmes B M
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107752
Published
1974-07-00
Pages
83-90
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC301526
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI107752 PMC Full Text View journal details
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