Involvement of the anticodon region of Escherichia coli tRNAGln and tRNAGlu in the specific interaction with cognate aminoacyl-tRNA synthetase. Alteration of the 2-thiouridine derivatives located in the anticodon of the tRNAs by BrCN or sulfur deprivation.

Adenosine Triphosphate/metabolism Amino Acyl-tRNA Synthetases/metabolism Carbon Radioisotopes Chromatography, Ion Exchange Cyanogen Bromide Diphosphates/metabolism Escherichia coli/drug effects,metabolism Glutamates Glutamine Kinetics Phosphorus Radioisotopes Protein Biosynthesis RNA, Bacterial/metabolism RNA, Transfer/metabolism Spectrophotometry, Ultraviolet Thiouridine/pharmacology Transfer RNA Aminoacylation

Carbon Radioisotopes Diphosphates Glutamates Phosphorus Radioisotopes RNA, Bacterial Glutamine Thiouridine Adenosine Triphosphate RNA, Transfer Amino Acyl-tRNA Synthetases Cyanogen Bromide