Home LiteratureArticle Details
PMID: 4367458 Published · ppublish English Journal Article

Biophysical-immunological assay for ribonucleic acid type C viruses.

Applied microbiology ·Vol. 28 ·No. 1 ·1974-07-00 ·Pages 100-5

Olpin J, Oroszlan S, Gilden RV

Abstract

A biophysical and immunological method for characterization of ribonucleic acid type C virus suspensions is described. The method provides a relationship to the total viral mass concentration of the particle titer, the gs antigen titer, and the ultraviolet absorbance (268 nm) of 2% sodium dodecyl sulfate digests. Data for murine, rat, feline, and hamster viruses are shown to be analogous within the test limitations. From these data, an assessment of the viral purity can be made, the structural integrity can be evaluated, an approximate molecular weight can be computed, and the mole ratio of gs antigen can be determined.

MeSH Terms
Animals Antigens, Viral/analysis Colorimetry Cricetinae Immunodiffusion Latex Leukemia Virus, Feline/immunology,isolation & purification Leukemia Virus, Murine/immunology,isolation & purification Methods Microscopy, Electron Microspheres Molecular Weight Oncogenic Viruses/immunology,isolation & purification Rats Retroviridae/immunology,isolation & purification Spectrophotometry, Ultraviolet
Chemicals
Antigens, Viral Latex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Olpin J
Oroszlan S
Gilden R V
References (22)
22 references, click to expand
  1. Avian tumor viruses.
    Adv Virus Res. 1965;11:293-385 PMID: 4285262
  2. Immunochemical quantitation of antigens by single radial immunodiffusion.
    Immunochemistry. 1965 Sep;2(3):235-54 PMID: 4956917
  3. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses.
    Cancer Res. 1967 Sep;27(9):1672-7 PMID: 6057678
  4. Biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (Moloney).
    J Virol. 1968 Sep;2(9):865-8 PMID: 5725318
  5. Interaction of Rous sarcoma virus with rat embryo fibroblasts of inbred Lewis strain in vitro.
    Folia Biol (Praha). 1968;14(6):457-65 PMID: 4303535
  6. Replication of cat leukemia virus in cell suspension cultures.
    Nature. 1969 May 10;222(5193):589-90 PMID: 5818677
  7. Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.
    Appl Microbiol. 1970 Aug;20(2):259-62 PMID: 4098102
  8. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus.
    Proc Natl Acad Sci U S A. 1971 May;68(5):901-4 PMID: 4102562
  9. Antigenic differentiation of M-MSV(O) from mouse, hamster, and cat C-type viruses.
    Virology. 1971 Mar;43(3):722-4 PMID: 4107555
  10. Phospholipid composition of Rous sarcoma virus, host cell membranes and other enveloped RNA viruses.
    Virology. 1971 Oct;46(1):106-16 PMID: 4108030
  11. Purification and immunological characterization of the major internal protein of the RD-114 virus.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1211-5 PMID: 4113865
  12. Determination of protein: a modification of the Lowry method that gives a linear photometric response.
    Anal Biochem. 1972 Aug;48(2):422-7 PMID: 4115981
  13. Karyotypic, virologic, and immunologic analyses of two continuous lymphocyte lines established from New Zealand black mice: possible relationship of chromosomal mosaicism to autoimmunity.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2965-9 PMID: 4342970
  14. Major group-specific protein of rat type C viruses.
    J Virol. 1972 Oct;10(4):746-50 PMID: 4117346
  15. Differential morphology of the RD virus from the human rhabdomyosarcoma, RD-114B cell line demonstrated by negative staining electron microscopy.
    Nat New Biol. 1972 Dec 13;240(102):212-4 PMID: 4118589
  16. Immunochemical quantitation of enzymes using multispecific antisera.
    Anal Biochem. 1972 Dec;50(2):344-53 PMID: 4118996
  17. A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymocyte serum.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):859-62 PMID: 4123693
  18. Isolation of a neurotropic type C virus.
    Science. 1973 Sep 7;181(4103):945-7 PMID: 4354228
  19. Spontaneous production of a C-type RNA virus in rat tissue culture lines.
    Int J Cancer. 1973 Nov 15;12(3):654-66 PMID: 4134008
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Virus of avian myeloblastosis. XII. Chemical constitution.
    J Natl Cancer Inst. 1959 Jul;23(1):183-97 PMID: 13673277
  22. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.
    J Immunol. 1965 Jan;94:84-90 PMID: 14253527
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.28.1.100-105.1974
Published
1974-07-00
Pages
100-5
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC186602
Subset
IM
External Links
PubMed source DOI: 10.1128/am.28.1.100-105.1974 PMC Full Text
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2