Isolation of synaptic plasma membrane from brain by combined flotation-sedimentation density gradient centrifugation.

Acetylcholinesterase/metabolism Adenosine Triphosphatases/metabolism Animals Brain/cytology,enzymology Cell Fractionation Cell Membrane Cell Nucleus/enzymology Centrifugation, Density Gradient Electron Transport Complex IV/metabolism Evaluation Studies as Topic Female Methods Mitochondria/enzymology Neurons/cytology Nucleotides Phosphoric Diester Hydrolases/metabolism Potassium Rats Sodium Subcellular Fractions/enzymology Synaptic Membranes

Nucleotides Sodium Electron Transport Complex IV Acetylcholinesterase Phosphoric Diester Hydrolases Adenosine Triphosphatases Potassium