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PMID: 4369261 Published · ppublish English Journal Article

Target cell polarity and membrane phosphorylation in relation to the mechanism of action of antidiuretic hormone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2595-9

Schwartz IL, Shlatz LJ, Kinne-Saffran E, Kinne R

Abstract

The plasma membrane of the bovine renal collecting duct epithelial cell has been resolved into its apical (luminal) and basal-lateral (contraluminal) components by free flow electrophoresis. The contraluminal, but not the luminal, membrane was found to contain antidiuretic hormone-sensitive adenylate cyclase. The luminal membrane was found to contain a cyclic 3':5'-adenosine monophosphate-sensitive self-phosphorylating system consisting of a membrane-bound protein kinase and its membrane-bound substrate(s); this intrinsic protein kinase was not present in the contraluminal membrane. These findings provide direct evidence that the initiating steps in the action of antidiuretic hormone on the kidney take place at the contraluminal pole of the hormonesensitive target cell and that the late or terminal steps occur at the luminal pole, where they involve an alteration in the level of membrane phosphorylation.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenylyl Cyclases/metabolism Animals Bicarbonates/pharmacology Calcium/pharmacology Carbon Radioisotopes Cattle Cell Fractionation Cell Membrane/enzymology Cell Membrane Permeability Cyclic AMP/metabolism Electrophoresis Enzyme Activation In Vitro Techniques Kidney/enzymology Phosphates/metabolism Phosphoric Diester Hydrolases/metabolism Protein Kinases/metabolism Receptors, Cell Surface Succinate Dehydrogenase/metabolism Vasopressins/metabolism,pharmacology
Chemicals
Bicarbonates Carbon Radioisotopes Phosphates Receptors, Cell Surface Vasopressins Cyclic AMP Succinate Dehydrogenase Protein Kinases Phosphoric Diester Hydrolases Adenosine Triphosphatases Adenylyl Cyclases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schwartz I L
Shlatz L J
Kinne-Saffran E
Kinne R
References (27)
27 references, click to expand
  1. The physiology of the urinary bladder of amphibia.
    Biol Rev Camb Philos Soc. 1966 May;41(2):275-316 PMID: 5295798
  2. Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules.
    Am J Physiol. 1966 Jul;211(1):255-9 PMID: 5911047
  3. Membrane effects of antidiuretic hormone.
    Am J Med. 1967 May;42(5):745-56 PMID: 5337376
  4. The role of adenosine 3',5'-phosphate in the action of antidiuretic hormone.
    Am J Med. 1967 May;42(5):757-68 PMID: 5337377
  5. Ultrastructural studies of vasopressin effect on isolated perfused renal collecting tubules of the rabbit.
    J Cell Biol. 1968 Feb;36(2):355-67 PMID: 4867134
  6. Adenyl cyclase assay in fat cell ghosts.
    Anal Biochem. 1969 Jun;29(3):476-89 PMID: 4307204
  7. [8-Arginine]-vasopressinoic acid: an inhibitor of rabbit kidney adenyl cyclase.
    Science. 1970 Feb 20;167(3921):1134-5 PMID: 4983621
  8. A cyclic AMP-dependent protein kinase from frog bladder epithelial cells.
    Biochem Biophys Res Commun. 1970 May 22;39(4):559-66 PMID: 4321408
  9. Cyclic nucleotide-dependent protein kinases. V. Preparation and properties of adenosine 3',5'-monophosphate-dependent protein kinase from various bovine tissues.
    Biochim Biophys Acta. 1970 Jul 15;212(1):79-91 PMID: 4322697
  10. Factors involved in the action of cyclic AMP on the permeability of mammalian kidney and toad urinary bladder.
    Ann N Y Acad Sci. 1971 Dec 30;185:345-50 PMID: 4330500
  11. Properties of soluble ATPase of gastric mucosa. I. Effect of HCO 3 -.
    Biochim Biophys Acta. 1971 Oct 12;249(1):101-13 PMID: 4258791
  12. Cyclic 3',5'-AMP-dependent protein kinase activity in toad bladder epithelium.
    Proc Soc Exp Biol Med. 1972 Jun;140(2):657-60 PMID: 4338801
  13. Characterization of the adenyl cyclase of rat kidney plasma membranes.
    Biochim Biophys Acta. 1972 May 9;266(2):524-42 PMID: 4338880
  14. The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings.
    J Cell Biol. 1972 Aug;54(2):232-45 PMID: 4261147
  15. Cyclic AMP-dependent reversible phosphorylation of renal medullary plasma membrane protein.
    Endocrinology. 1972 Sep;91(3):757-63 PMID: 4339330
  16. Studies on the mechanism of hormone action.
    Science. 1972 Aug 4;177(4047):401-8 PMID: 4339614
  17. Preparation and characterization of a hormone-responsive renal plasma membrane fraction.
    J Biol Chem. 1972 Nov 10;247(21):6913-8 PMID: 4263617
  18. Kidney membrane cyclic AMP receptor and cyclic AMP-dependent protein kinase activities: comparison of plasma membrane and cytoplasmic fractions.
    Biochem Biophys Res Commun. 1972 Dec 18;49(6):1510-7 PMID: 4344812
  19. Calcium-mediated interactions between the antidiuretic hormone and renal plasma membranes.
    J Biol Chem. 1972 Oct 10;247(19):6167-75 PMID: 4346807
  20. Regulation of phosphorylation of a specific protein in toad-bladder membrane by antidiuretic hormone and cyclic AMP, and its possible relationship to membrane permeability changes.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):880-4 PMID: 4351809
  21. The vasopressin-sensitive adenylate cyclase of the rat renal medulla.
    J Biol Chem. 1973 Jul 10;248(13):4775-81 PMID: 4352409
  22. Activation by adenosine 3':5'-monophosphate of a membrane-bound phosphoprotein phosphatase from toad bladder.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1831-5 PMID: 4352657
  23. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  24. [Renal tubular hydrogen-ion secretion in mammals].
    Pflugers Arch. 1958;267(5):491-6 PMID: 13601055
  25. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  26. Structure of the toad's urinary bladder as related to its physiology.
    J Biophys Biochem Cytol. 1961 Aug;10:529-53 PMID: 13733732
  27. QUANTITATIVE MICRODETERMINATION OF ENZYMES IN SWEAT GLAND. 3. SUCCINIC DEHYDROGENASE IN CYSTIC FIBROSIS.
    Proc Soc Exp Biol Med. 1965 Jun;119:470-3 PMID: 14328921
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.7.2595
Published
1974-07-00
Pages
2595-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388512
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.7.2595 PMC Full Text View journal details
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