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PMID: 4370520 Published · ppublish English Journal Article

Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3129-33

Chatterjee NK, Weissbach H

Abstract

Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but evidence is presented that the nuclei are involved in the conversion of high-molecular-weight RNA to low-molecular-weight species. Some of the newly synthesized polypeptides appear to resemble the virion polypeptides.

MeSH Terms
Adenoviridae/metabolism Animals Carbon Radioisotopes Carcinoma, Ehrlich Tumor/metabolism Cell Nucleus/metabolism Cell-Free System Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Female HeLa Cells/metabolism Humans Leucine/metabolism Methionine/metabolism Molecular Weight Peptide Biosynthesis Precipitin Tests Protein Biosynthesis RNA/metabolism RNA, Neoplasm/metabolism RNA, Viral/biosynthesis Simian virus 40 Tritium Viral Proteins/biosynthesis
Chemicals
Carbon Radioisotopes RNA, Neoplasm RNA, Viral Viral Proteins Tritium RNA Methionine Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chatterjee N K
Weissbach H
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.8.3129
Published
1974-08-00
Pages
3129-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388635
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.8.3129 PMC Full Text View journal details
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