Home LiteratureArticle Details
PMID: 4372633 Published · ppublish English Journal Article

13C nuclear magnetic resonance spectroscopic evidence for hydrophobic lipid-protein interactions in human high density lipoproteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3696-700

Stoffel W, Zierenberg O, Tunggal B, Schreiber E

Abstract

Phosphatidylcholines, sphingomyelins, cholesterol, and cholesterol esters were enriched with (13)C by chemical synthesis in specific positions of their hydrophilic groups and aliphatic chains. Their spin-lattice relaxation times were determined in organic solvents. The substances were organized as liposomes and recombined with total human high density apolipoproteins and the two separated main components, apolipoprotein A-I (apoLp-Gln-I) and apolipoprotein A-II (apoLp-Gln-II). These (13)C nuclear magnetic resonance data established that in reassembled high density lipoproteins the phospholipid molecules bind to the apoprotein moieties with their hydrophobic fatty acid chains and not with their hydrophilic zwitterionic groups. Apolipoprotein A-I preferentially binds phosphatidylcholine, although its lipid-binding capacity is smaller than that of apolipoprotein A-II. Apolipoprotein A-II avidly reassembles with sphingomyelin by hydrophobic interactions. A model of the molecular organization of the high density lipoportein particle has been derived.

MeSH Terms
Apoproteins Carbon Radioisotopes Cholesterol Esters Fatty Acids Lipids Lipoproteins, HDL Magnetic Resonance Spectroscopy Models, Molecular Phosphatidylcholines Protein Conformation Proteins Sphingomyelins Water
Chemicals
Apoproteins Carbon Radioisotopes Esters Fatty Acids Lipids Lipoproteins, HDL Phosphatidylcholines Proteins Sphingomyelins Water Cholesterol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stoffel W
Zierenberg O
Tunggal B
Schreiber E
References (37)
37 references, click to expand
  1. Quantitative analysis of phospholipids by thin-layer chromatography.
    Biochem J. 1964 Feb;90(2):374-8 PMID: 4284220
  2. Separation of lipid classes by thin-layer chromatography.
    Biochim Biophys Acta. 1965 Oct 4;106(2):386-96 PMID: 5867698
  3. Electrophoretic heterogeneity of polypeptide chains of specific antibodies.
    Science. 1966 May 27;152(3726):1253-5 PMID: 4160819
  4. Forms of human serum high density lipoprotein protein.
    J Lipid Res. 1966 Mar;7(2):295-306 PMID: 4957797
  5. Binding of human serum high density lipoprotein apoprotein with aqueous dispersions of phospholipids.
    J Biol Chem. 1967 Feb 25;242(4):711-9 PMID: 6017739
  6. On the structure of human serum high-density lipoprotein: studies by the technique of circular dichroism.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):890-4 PMID: 5238669
  7. Molecular weight and subunit structure of human serum high density lipoproteinafter chemical modifications by succinic anhydride.
    Biochim Biophys Acta. 1968 May 6;160(1):32-45 PMID: 5651693
  8. Heterogeneity in protein subunits of human serum high-density lipoproteins.
    Biochemistry. 1968 Aug;7(8):2773-7 PMID: 5666751
  9. Structure of human serum lipoproteins: nuclear magnetic resonance supports a micellar model.
    Science. 1968 Nov 22;162(3856):909-11 PMID: 5684497
  10. Studies on phosphatidylcholine vesicles. Formation and physical characteristics.
    Biochemistry. 1969 Jan;8(1):344-52 PMID: 5777332
  11. The structure of human density lipoprotein: a study of the effect of phospholipase A and trypsin on its components and of the behavior of the lipid and protein moieties at the air-water interphase.
    Biochim Biophys Acta. 1969 Mar;175(2):290-300 PMID: 5778170
  12. Nuclear magnetic resonance studies on serum low density lipoproteins (LDL2).
    Chem Phys Lipids. 1969 Apr;3(2):152-8 PMID: 5784125
  13. High-resolution NMR spectra of high-density serum lipoproteins.
    Biochim Biophys Acta. 1969 Apr 29;176(3):524-36 PMID: 5800042
  14. Fractionation of human serum high density lipoprotein in urea solutions. Evidence for polypeptide heterogeneity.
    Biochemistry. 1969 Aug;8(8):3309-16 PMID: 4980192
  15. Isolation and characterization of polypeptides of human serum lipoproteins.
    Biochemistry. 1969 Nov;8(11):4510-6 PMID: 5353112
  16. Monolayer studies with synthetic saturated, mono- and polyunsaturated mixed 1,2-diglycerides, 1,2-diacylphosphatidylethanolamines and phosphatidylcholines at the air-water-interface.
    Hoppe Seylers Z Physiol Chem. 1969 Nov;350(11):1385-93 PMID: 5362611
  17. Recent studies on the structure of human serum low-and high-density lipoproteins.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1119-27 PMID: 5264142
  18. Functions and interrelationships of different classes of plasma lipoproteins.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1128-37 PMID: 5264143
  19. A simple chemical method for labelling phosphatidylcholine and sphingomyelin in the choline moiety.
    Hoppe Seylers Z Physiol Chem. 1971 Aug;352(8):1058-64 PMID: 5098348
  20. Studies on the state of phosphatidylcholine molecules before and after ultrasonic and gel-filtration treatments.
    Biochem Biophys Res Commun. 1972 Feb 25;46(4):1660-6 PMID: 5062741
  21. On the metabolism of sphinganyl- and sphingenyl-1-phosphorylcholine.
    Hoppe Seylers Z Physiol Chem. 1972 Jan;353(1):65-74 PMID: 4335782
  22. The influence of lipid on the conformation of human plasma high density apolipoproteins.
    J Biol Chem. 1972 Apr 25;247(8):2598-606 PMID: 4336379
  23. 13 C nuclear magnetic resonance relaxation measurements of synthetic lecithins and the effect of spin-labeled lipids.
    Biochemistry. 1972 Apr 11;11(8):1416-21 PMID: 5021597
  24. Amino acid sequence of human apoLp-Gln-II (apoA-II), an apolipoprotein isolated from the high-density lipoprotein complex.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1304-8 PMID: 4338591
  25. Studies on the protein defect in Tangier disease. Isolation and characterization of an abnormal high density lipoprotein.
    J Clin Invest. 1972 Oct;51(10):2505-19 PMID: 4341435
  26. On the subunit structure of the protein of human serum high density lipoprotein. I. A study of its major polypeptide component (Sephadex, fraction 3).
    J Biol Chem. 1972 Sep 25;247(18):5842-9 PMID: 4341494
  27. Isolation and characterization of apoLp-Gln-II (apoA-II), a plasma high density apolipoprotein containing two identical polypeptide chains.
    J Biol Chem. 1972 Dec 10;247(23):7510-8 PMID: 4344224
  28. Application of electron microscopy to the study of plasma lipoprotein structure.
    Adv Lipid Res. 1972;10:1-41 PMID: 4344798
  29. Differences between conformations of lecithin and phosphatidylethanolamine polar groups and their effects on interactions of phospholipid bilayer membranes.
    Biochim Biophys Acta. 1972 Dec 1;290(1):397-402 PMID: 4640771
  30. 13 C-nuclear magnetic resonance spectroscopic studies on saturated, mono-, di- and polyunsaturated fatty acids, phospho- and sphingolipids.
    Hoppe Seylers Z Physiol Chem. 1972 Dec;353(12):1962-9 PMID: 4650175
  31. A study of the cystine-containing apolipoprotein of human plasma high density lipoproteins: characterization of cyanogen bromide and tryptic fragments.
    Biochim Biophys Acta. 1972 Nov 28;285(1):36-47 PMID: 4347238
  32. Small-angle x-ray scattering of human serum high-density lipoproteins.
    J Supramol Struct. 1972;1(2):98-104 PMID: 4347292
  33. Effect of sonication on the structure of lecithin bilayers.
    Biochemistry. 1972 Nov 21;11(24):4573-81 PMID: 4654145
  34. Natural abundance carbon-13 nuclear magnetic resonance spectra of human serum lipoproteins.
    Science. 1973 Apr 15;180(4082):193-5 PMID: 4348464
  35. Studies on the structure of lipoprotein A of human high density lipoprotein HDL3: the spherically averaged electron density distribution.
    FEBS Lett. 1973 Jun 15;33(1):77-80 PMID: 4352937
  36. Location of aromatic amino acid residues in bovine serum high-density lipoprotein.
    Biochemistry. 1973 Oct 23;12(22):4503-7 PMID: 4356242
  37. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3696
Published
1974-09-00
Pages
3696-700
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433843
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3696 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2