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PMID: 4375970 Published · ppublish English Journal Article

The kinetics of coupled enzyme reactions. Applications to the assay of glucokinase, with glucose 6-phosphate dehydrogenase as coupling enzyme.

The Biochemical journal ·Vol. 141 ·No. 1 ·1974-07-00 ·Pages 205-9

Storer AC, Cornish-Bowden A

Abstract

1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that the second reaction follows first-order kinetics. 2. A simple procedure is described for applying the theory to the practical design of enzyme assays. 3. The validity of the theory is confirmed for the assay of glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. The possibility of extending the theory to three or more coupled reactions is discussed.

MeSH Terms
Animals Chromatography, Affinity Enzymes Glucokinase/analysis Glucosephosphate Dehydrogenase Kinetics Models, Chemical NAD Rats Spectrophotometry
Chemicals
Enzymes NAD Glucosephosphate Dehydrogenase Glucokinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Storer A C
Cornish-Bowden A
References (12)
12 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1410205
Published
1974-07-00
Pages
205-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168067
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1410205 PMC Full Text View journal details
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