Kinetic studies of bovine liver carbamoyl phosphate synthetase.
A through study of initial-rate data has been made on carbamoyl phosphate synthetase from bovine liver. On the basis of the results the order of substrate binding to the enzyme is ATPMg followed by HCO(3) (-), ATPMg and NH(4) (+). A model for the enzymic mechanism is proposed, and the rate equations describing it are presented. Details of the derivation of the initial-rate equation for the kinetic mechanism proposed have been deposited as Supplementary Publication SUP 50032 (6 pages) at the British Library, Lending Division (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7QB, U.K., from whom copies may be obtained on the terms indicated in Biochem. J. (1973), 131, 5.
13 references, click to expand
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A simple method for derivation of rate equations for enzyme-catalyzed reactions under the rapid equilibrium assumption or combined assumptions of equilibrium and steady state.J Biol Chem. 1968 Feb 25;243(4):820-5 PMID: 5638598
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Kinetic studies on rat liver carbamyl phosphate synthetase.J Biol Chem. 1968 Aug 25;243(16):4279-85 PMID: 5679964
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Structure and function of carbamoylphosphate synthase. I. Transitions between two catalytically inactive forms and the active form.Eur J Biochem. 1968 Dec;7(1):119-27 PMID: 5751125
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Structure and function of carbamoylphosphate synthetase. On the mechanism of bicarbonate activation.Eur J Biochem. 1969 Mar;8(2):207-14 PMID: 5781273
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Purification and characterisation of carbamoyl phosphate synthetase from beef liver.FEBS Lett. 1973 Nov 15;37(1):79-81 PMID: 4753763
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A kinetic analysis of enzyme systems involving four substrates.Biochem J. 1974 Sep;141(3):789-805 PMID: 4463962
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STUDIES ON THE MECHANISM OF ACTION OF ACETYLGLUTAMATE AS AN ACTIVATOR OF CARBAMYL PHOSPHATE SYNTHETASE.J Biol Chem. 1964 Jun;239:1935-41 PMID: 14213380
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Studies on the biosynthesis of carbamyl phosphate.J Biol Chem. 1957 Dec;229(2):1019-25 PMID: 13502361
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Isolation and characterization of a naturally occurring cofactor of carbamyl phosphate biosynthesis.J Biol Chem. 1958 Feb;230(2):1013-21 PMID: 13525417
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Purification of carbamyl phosphate synthetase from frog liver.J Biol Chem. 1958 Jul;233(1):102-5 PMID: 13563449
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Formation constants for the complexes of adenosine di- or tri-phosphate with magnesium or calcium ions.Biochem J. 1959 Feb;71(2):388-95 PMID: 13628582
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A KINETIC STUDY OF CARBAMYL PHOSPHATE SYNTHETASE.J Biol Chem. 1964 Jun;239:1925-34 PMID: 14213379
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Product inhibition studies on bovine liver carbamoyl phosphate synthetase.Biochem J. 1974 Sep;141(3):817-24 PMID: 4377108
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