Direct evidence for Hageman factor (factor XII) activation by bacterial lipopolysaccharides (endotoxins).

The Journal of experimental medicine ·Vol. 140 ·No. 3 ·1974-09-01 ·Pages 797-811

Morrison DC, Cochrane CG

Abstract

Purified precursor Hageman factor has been demonstrated to bind to soluble bacterial lipopolysaccharide (LPS, endotoxin) isolated from Escherichia coli 0111:B4, and this complex has been shown to have the capacity to convert prekallikrein to its active form. In addition, LPS-activated Hageman factor substantially reduces clotting times in XII-deficient plasma. The capacity to activate Hageman factor has been demonstrated to reside in the lipid A region of the LPS molecule. Activation of Hageman factor by LPS contrasts with fluid-phase activation (e.g., by kallikrein or trypsin) in that no cleavage to lower molecular weight fragments occurs. High concentrations of LPS inhibit the activity of Hageman factor, probably by a direct LPS-Hageman factor interaction.

MeSH Terms

Animals Blood Coagulation Endotoxins Enzyme Activation Enzyme Precursors Escherichia coli/immunology Factor XII/metabolism Humans Iodine Radioisotopes Kallikreins/biosynthesis Polysaccharides, Bacterial/metabolism Protein Binding Rabbits Salmonella/immunology

Chemicals

Endotoxins Enzyme Precursors Iodine Radioisotopes Polysaccharides, Bacterial Factor XII Kallikreins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Morrison D C

Cochrane C G

References (25)

25 references, click to expand

Activation of Hagemen Factor by saturated fatty acids.
Aust J Exp Biol Med Sci. 1962 Dec;40:505-13 PMID: 13933027
A method of trace iodination of proteins for immunologic studies.
Int Arch Allergy Appl Immunol. 1966;29(2):185-9 PMID: 4160044
Consumption of Hageman factor activity in the generalized Shwartzman reaction induced by liquoid. Its prevention by inhibition of Hageman factor activation.
Thromb Diath Haemorrh. 1970 May 31;23(2):386-404 PMID: 5426216
A new method for the extraction of R lipopolysaccharides.
Eur J Biochem. 1969 Jun;9(2):245-9 PMID: 5804498
Endotoxin-induced intravascular clotting: the need for granulocytes.
Thromb Diath Haemorrh. 1968 Dec 31;20(3):430-43 PMID: 5751635
ACTINOMYCIN SENSITIVITY IN ESCHERICHIA COLI PRODUCED BY EDTA.
Biochem Biophys Res Commun. 1965 Jan 4;18:13-7 PMID: 14265747
Activation of Hageman factor by ellagic acid and the generalized Shwartzman reaction.
Am J Pathol. 1969 Mar;54(3):393-420 PMID: 4975375
The Origin of the Epithelioid Cell in Experimental Tuberculosis of the Cornea.
Am J Pathol. 1933 May;9(3):337-346.9 PMID: 19970073
Anticomplementary activity of lipid A isolated from lipopolysaccharides.
Proc Soc Exp Biol Med. 1973 Sep;143(4):1025-30 PMID: 4582770
Chemical, physical, biological properties of a lipopolysaccharide from Escherichia coli K-235.
Biochemistry. 1967 Aug;6(8):2363-72 PMID: 4867999
The role of Hageman factor in the initiation of blood coagulation.
Br J Haematol. 1959 Apr;5(2):203-11 PMID: 13651548
Estimation of 3-deoxy sugars by means of the malonaldehyde-thiobarbituric acid reaction.
Nature. 1960 Apr 9;186:155-6 PMID: 13813473
Pathophysiology of disseminated intravascular coagulation.
Thromb Diath Haemorrh Suppl. 1969;36:45-61 PMID: 4316397
Plasma kallikrein and Hageman factor in Gram-negative bacteremia.
Ann Intern Med. 1970 Oct;73(4):545-51 PMID: 5506006
Nature and linkages of the fatty acids present in the lipid-A component of Salmonella lipopolysaccharides.
Eur J Biochem. 1972 Jul 13;28(2):166-73 PMID: 5069711
Activation of Hageman factor in solid and fluid phases. A critical role of kallikrein.
J Exp Med. 1973 Dec 1;138(6):1564-83 PMID: 4271629
THE CLOT-PROMOTING EFFECT OF SOAPS OF LONG-CHAIN SATURATED FATTY ACIDS.
J Clin Invest. 1963 Oct;42:1569-77 PMID: 14074352
Intravascular clotting after endotoxin in rabbits with impaired intrinsic clotting produced by a factor VIII antibody.
Blood. 1973 Oct;42(4):523-34 PMID: 4778703
The first component of the kinin-forming system in human and rabbit plasma. Its relationship to clotting factor XII (Hageman Factor).
J Exp Med. 1971 Oct 1;134(4):986-1004 PMID: 4106492
Induction of disseminated intravascular coagulation in the factor XII-deficient fowl. Morphological effects of liquoid, bacterial endotoxin and tissue thromboplastin in the normal and anticoagulated fowl.
Thromb Diath Haemorrh. 1973 Sep 15;30(1):25-35 PMID: 4788751
Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2129-33 PMID: 4579014
ACTIVATION OF HAGEMAN FACTOR (FACTOR XII) BY LONG-CHAIN SATURATED FATTY ACIDS.
Thromb Diath Haemorrh. 1963 Jul 15;33:346-53 PMID: 14085152
Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate.
J Biol Chem. 1968 Dec 25;243(24):6384-91 PMID: 4973230
Endotoxin-induced kinin production in man.
Proc Soc Exp Biol Med. 1972 Mar;139(3):1078-82 PMID: 5023759
Hageman factor activation in the generalized Shwartzman reaction induced by endotoxin.
Br J Haematol. 1971 Nov;21(5):513-27 PMID: 5111702

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.140.3.797

Published

1974-09-01

Pages

797-811

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139619

Subset

External Links

PubMed source DOI: 10.1084/jem.140.3.797 PMC Full Text View journal details

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Direct evidence for Hageman factor (factor XII) activation by bacterial lipopolysaccharides (endotoxins).

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