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PMID: 4379260 Published · ppublish English Journal Article

Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.

The Journal of cell biology ·Vol. 26 ·No. 1 ·1965-07-00 ·Pages 219-43

Baudhuin P, Beaufay H, De Duve C

Abstract

Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.

MeSH Terms
Animals Biochemical Phenomena Biochemistry Catalase/metabolism Chemistry Techniques, Analytical D-Amino-Acid Oxidase/metabolism In Vitro Techniques Liver/cytology,enzymology Lysosomes Microscopy, Electron Rats Ultracentrifugation Urate Oxidase/metabolism
Chemicals
Catalase D-Amino-Acid Oxidase Urate Oxidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baudhuin P
Beaufay H
De Duve C
References (21)
21 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.26.1.219
Published
1965-07-00
Pages
219-43
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2106697
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.26.1.219 PMC Full Text View journal details
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