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PMID: 4384595 Published · ppublish English Journal Article

Effect of thyroidectomy on pathways of glucose metabolism in lactating rat mammary gland.

The Biochemical journal ·Vol. 105 ·No. 2 ·1967-11-00 ·Pages 615-23

Walters E, McLean P

Abstract

1. Assessment of the overall metabolic changes in lactating mammary gland after thyroidectomy has been made by measurement of the incorporation of (14)C from specifically labelled glucose, pyruvate and acetate into (14)CO(2) and (14)C-labelled lipid in the experimental rats and in sham-operated control animals. 2. Thyroidectomy depressed the oxidation of (14)C-labelled substrates, an effect still apparent when the control rats were pair-fed with thyroidectomized rats; however, the ratio of oxidation of [1-(14)C]glucose/oxidation of [6-(14)C]glucose was unaltered. In parallel with these studies it was revealed that the activities of hexokinase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and NADP-linked isocitrate dehydrogenase were all lower in the thyroidectomized group than in the pair-fed control group. 3. Thyroidectomy also lowered the incorporation of (14)C-labelled substrates into (14)C-labelled lipid, an effect further studied by measurement of the activities of citrate-cleavage enzyme and acetate thiokinase. Restricting the food intake of the control rats to that of the thyroidectomized group lowered the activity of citrate-cleavage enzyme, but no further depression was observed on thyroidectomy. The oxidized and reduced nicotinamide nucleotide content of mammary tissue was shown to be decreased in the thyroidectomized rats compared with the control rats.

MeSH Terms
Acetates/metabolism Alcohol Oxidoreductases/metabolism Animals Carbon Dioxide/metabolism Carbon Isotopes Female Glucose/metabolism Growth Lactation Lipid Metabolism Mammary Glands, Animal/metabolism NAD/metabolism NADP/metabolism Nucleic Acids/metabolism Oxidation-Reduction Phosphotransferases/metabolism Pregnancy Pyruvates/metabolism Rats Thyroidectomy
Chemicals
Acetates Carbon Isotopes Nucleic Acids Pyruvates NAD Carbon Dioxide NADP Alcohol Oxidoreductases Phosphotransferases Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Walters E
McLean P
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38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1050615
Published
1967-11-00
Pages
615-23
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198353
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1050615 PMC Full Text View journal details
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