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PMID: 4386930 Published · ppublish English Journal Article

The isolation and characterization of 3-phosphoglycerate dehydrogenase from peas.

The Biochemical journal ·Vol. 109 ·No. 5 ·1968-10-00 ·Pages 743-8

Slaughter JC, Davies DD

Abstract

1. 3-Phosphoglycerate dehydrogenase was purified 400-fold from crude extracts of etiolated pea epicotyls. 2. Michaelis constants were determined for all four substrates. 3. Loss of sensitivity to inhibition by l-serine occurs on purification. 4. The purified enzyme is inhibited by thiol-group reagents and, with N-ethyl-maleimide, protection is afforded by 3-phosphoglycerate though not by NAD(+).

MeSH Terms
Alcohol Oxidoreductases Ethylmaleimide/pharmacology Glycerophosphates Hydrogen-Ion Concentration Kinetics NAD/pharmacology NADP/pharmacology Oxidoreductases/antagonists & inhibitors Plants/enzymology Serine/pharmacology
Chemicals
Glycerophosphates NAD Serine NADP Oxidoreductases Alcohol Oxidoreductases Ethylmaleimide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Slaughter J C
Davies D D
References (11)
11 references, click to expand
  1. Purification and properties of chicken liver D-3-phosphoglycerate dehydrogenase.
    Biochemistry. 1965 Jun;4(6):1076-85 PMID: 4378782
  2. The biosynthesis of serine in mouse brain extracts.
    J Biol Chem. 1965 Dec;240(12):4591-7 PMID: 4284887
  3. Comparative studies on the pathways for serine biosynthesis in animal tissues.
    J Biol Chem. 1966 Sep 10;241(17):4068-76 PMID: 5920812
  4. Synthesis of serine in the liver of vertebrates.
    Comp Biochem Physiol. 1966 Nov;19(3):589-96 PMID: 6008344
  5. Nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Purification and properties.
    Biochem J. 1967 Nov;105(2):729-34 PMID: 4296325
  6. Inhibition of 3-phosphoglycerate dehydrogenase by l-serine.
    Biochem J. 1968 Oct;109(5):749-55 PMID: 5696864
  7. The enzymatic reduction of hydroxypyruvic acid to D-glyceric acid in higher plants.
    J Biol Chem. 1954 Apr;207(2):621-9 PMID: 13163046
  8. The stereospecificity of enzymatic hydrogen transfer from diphosphopyridine nucleotide.
    J Biol Chem. 1957 Sep;228(1):85-96 PMID: 13475298
  9. Chromatography of some 2,4-dinitrophenylhydrazones in acid systems.
    J Chromatogr. 1962 Aug;8:433-41 PMID: 13875128
  10. THE PATHWAY AND CONTROL OF SERINE BIOSYNTHESIS IN ESCHERICHIA COLI.
    J Biol Chem. 1963 Dec;238:3934-44 PMID: 14086727
  11. ENZYMOLOGY AND REGULATION OF SERINE BIOSYNTHESIS IN CULTURED HUMAN CELLS.
    J Biol Chem. 1964 Dec;239:4219-26 PMID: 14247673
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1090743
Published
1968-10-00
Pages
743-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187024
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1090743 PMC Full Text View journal details
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