Number of transformable units per cell in Diplococcus pneumoniae.

Journal of bacteriology ·Vol. 97 ·No. 3 ·1969-03-00 ·Pages 1033-5

Porter RD, Guild WR

Abstract

Analysis of frequencies of single and random multiple transformations in Diplococcus pneumoniae showed that there are at least two transformable units per cell of the total population in highly competent cultures. If 100% of the cells are competent in these cases, the units may be interpreted as the strands of one duplex deoxyribonucleic acid recipient chromosome. The theory is developed to allow for extension to more complex situations.

MeSH Terms

Chromosomes, Bacterial Models, Theoretical Streptococcus pneumoniae Transformation, Genetic

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Porter R D

Guild W R

References (11)

11 references, click to expand

Studies on transformations of Hemophilus influenzae. I. Competence.
J Gen Physiol. 1961 Jul;44:1201-27 PMID: 13707010
Transformation and DNA size. I. Activity of fragments of defined size and a fit to a random double cross-over model.
J Mol Biol. 1968 Oct 14;37(1):157-78 PMID: 4398726
Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients.
J Bacteriol. 1968 Mar;95(3):867-75 PMID: 4966830
Purification of competent cells in the Bacillus subtilis transformation system.
J Bacteriol. 1968 Mar;95(3):876-85 PMID: 4966831
An autoradiographic study of genetic transformation.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1216-22 PMID: 4386320
Competence and deoxyribonucleic acid uptake in Bacillus subtilis.
J Bacteriol. 1968 Mar;95(3):864-6 PMID: 4966829
Fractionated strands of bacterial deoxyribonucleic acid. 3. Transformation efficiencies and rates of phenotypic expression.
J Bacteriol. 1968 Dec;96(6):1991-6 PMID: 4387147
Transmission of genetic information during transformation in Diplococcus pneumoniae.
Biochem Biophys Res Commun. 1968 Mar 27;30(6):683-9 PMID: 4384456
ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.
Proc Natl Acad Sci U S A. 1964 Aug;52:412-9 PMID: 14206608
Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA.
Science. 1967 Aug 18;157(3790):817-9 PMID: 17842787
Evidence for message reading from a unique strand of pneumococcal DNA.
Proc Natl Acad Sci U S A. 1963 Jul;50:106-12 PMID: 13951226

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.97.3.1033-1035.1969

Published

1969-03-00

Pages

1033-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249810

Subset

External Links

PubMed source DOI: 10.1128/jb.97.3.1033-1035.1969 PMC Full Text View journal details

Analysis Services

Number of transformable units per cell in Diplococcus pneumoniae.

Contact

WeChat Official Account