Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells.

Applied microbiology ·Vol. 17 ·No. 3 ·1969-03-00 ·Pages 422-6

Baker PJ, Stashak PW, Prescott B

Abstract

A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent. Erythrocytes so sensitized can be used in routine passive hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody and specific antibody-producing cells, respectively.

MeSH Terms

Animals Antibodies/analysis Antibody Formation Erythrocytes/immunology Female Hemagglutination Tests Hemolysis Mice Polysaccharides, Bacterial Spleen/immunology Streptococcus pneumoniae/immunology

Chemicals

Antibodies Polysaccharides, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Baker P J

Stashak P W

Prescott B

References (12)

12 references, click to expand

BREAKING OF IMMUNOLOGICAL PARALYSIS BY INJECTION OF A SPECIFIC DEPOLYMERASE.
Nature. 1964 Dec 26;204:1319-20 PMID: 14254431
Studies on the O antigen of Salmonella typhosa. III. Activity of the isolated antigen in the hemagglutination procedure.
Am J Hyg. 1955 Jul;62(1):54-65 PMID: 14398687
Chromic chloride: a coupling reagent for passive hemagglutination reactions.
J Immunol. 1967 Nov;99(5):859-66 PMID: 4169033
SEROLOGIC PROPERTIES OF BENTONITE PARTICLES COATED WITH MICROBIAL POLYSACCHARIDES.
Proc Soc Exp Biol Med. 1963 Nov;114:530-6 PMID: 14101233
The adsorption of bacterial polysaccharides by erythrocytes.
Immunology. 1958 Apr;1(2):157-71 PMID: 13538532
The use of spiral loops in serological and virological micro-methods.
Acta Microbiol Acad Sci Hung. 1955;3(1-2):191-202 PMID: 13301496
Studies on the mechanism of the immunological paralysis induced in mice by pneumococcal polysaccharides.
J Immunol. 1955 Jan;74(1):17-26 PMID: 13233513
A hemagglutination test and a specific depolymerase for studying immunologic paralysis in mice.
J Immunol. 1966 Feb;96(2):358-63 PMID: 4379854
Bacterial hemagglutination and hemolysis.
Bacteriol Rev. 1956 Sep;20(3):166-88 PMID: 13363771
Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis.
J Exp Med. 1965 Sep 1;122(3):483-504 PMID: 5839282
Studies on the induction, specificity, prevention and breaking of immunologic paralysis and immunity to pneumococcal polysaccharide.
J Immunol. 1966 Feb;96(2):364-72 PMID: 4379855
The significance of multiple antibody components in serum of immunized rabbits.
Immunology. 1960 Oct;3:336-51 PMID: 13684803

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.17.3.422-426.1969

Published

1969-03-00

Pages

422-6

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377705

Subset

External Links

PubMed source DOI: 10.1128/am.17.3.422-426.1969 PMC Full Text

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Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells.

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