Increased vascular permeability produced by human platelet granule cationic extract.

The Journal of clinical investigation ·Vol. 49 ·No. 2 ·1970-02-00 ·Pages 274-81

Nachman RL, Weksler B, Ferris B

Abstract

A cationic protein extract obtained from isolated human platelet granules increased vascular permeability in mouse and rabbit skin. The permeability-enhancing effect was not inhibited by soybean trypsin and pancreatic trypsin inhibitor, methylsergide maleate, carboxypeptidase B, and C[unk]1 inactivator. Permeability-enhancing activity was blocked by prior treatment of challenged animals with antihistamine. The nondializable relatively heat-stable cationic granule protein extract possessed potent mastocytolytic activity. The experiments described suggest that human platelets exert a permeability-enhancing effect by lysosomal release of cationic proteins which cause histamine release from adjacent tissue mast cells.

MeSH Terms

Animals Blood Platelets/analysis Carboxypeptidases/pharmacology Cell Membrane Permeability/drug effects Electrophoresis Histamine H1 Antagonists/pharmacology Humans Ions Lysosomes/analysis Maleates/pharmacology Mast Cells/drug effects Methysergide/pharmacology Mice Proteins/antagonists & inhibitors,pharmacology Rabbits Skin/blood supply Soybeans Tissue Extracts/pharmacology Trypsin/pharmacology Trypsin Inhibitors/pharmacology

Chemicals

Histamine H1 Antagonists Ions Maleates Proteins Tissue Extracts Trypsin Inhibitors Carboxypeptidases Trypsin Methysergide

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nachman R L

Weksler B

Ferris B

References (26)

26 references, click to expand

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Article Info

Journal

The Journal of clinical investigation

Abbr.

J Clin Invest

ISSN

0021-9738

DOI

10.1172/JCI106237

Published

1970-02-00

Pages

274-81

Language

English

Region

United States

NLM ID

7802877

PMCID

PMC322470

Subset

External Links

PubMed source DOI: 10.1172/JCI106237 PMC Full Text View journal details

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Increased vascular permeability produced by human platelet granule cationic extract.

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