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PMID: 4394999 Published · ppublish English Journal Article

Quantitative and qualitative microbiological profiles of the Apollo 10 and 11 spacecraft.

Applied microbiology ·Vol. 20 ·No. 3 ·1970-09-00 ·Pages 384-9

Puleo JR, Oxborrow GS, Fields ND, Hall HE

Abstract

Microbiological profiles were determined for surfaces of the command module, lunar module (ascent and descent stages), instrument unit, Saturn S-4B stage, and the spacecraft lunar module adapter of the Apollo 10 and 11 spacecraft. Average levels of contamination of the command module were 2.1 x 10(4) and 2.7 x 10(4) microorganisms per ft(2) for Apollo 10 and 11, respectively. With the exception of the exterior surfaces of the ascent stage of the lunar module and the interior surfaces of the command module, average levels of microbial contamination on all components of the Apollo 11 were found to be lower than those observed on Apollo 10. For each Apollo mission, approximately 2,000 colonies were picked from a variety of media and identified. The results showed that approximately 95% of all isolates were those considered indigenous to humans; the remaining were associated with soil and dust in the environment. However, the ratio of these two general groups varied depending on the degrees of personnel density and environmental control associated with each module.

MeSH Terms
Bacteria/isolation & purification,pathogenicity Ecological Systems, Closed Fungi/isolation & purification,pathogenicity Humans Space Flight Yeasts/isolation & purification,pathogenicity
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Puleo J R
Oxborrow G S
Fields N D
Hall H E
References (4)
4 references, click to expand
  1. Feasibility of using ultrasonics for removing viable microorganisms from surfaces.
    Contam Control. 1967 Apr;6(4):58-62 passim PMID: 4227402
  2. Back contamination.
    Contam Control. 1968 Sep;7(9):19-23 PMID: 4236895
  3. Use of ultrasonic energy in assessing microbial contamination on surfaces.
    Appl Microbiol. 1967 Nov;15(6):1345-51 PMID: 16349743
  4. Lunar receiving laboratory.
    Science. 1967 Feb 3;155(3762):525-9 PMID: 17737398
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
Published
1970-09-00
Pages
384-9
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC376944
Subset
IM
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