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PMID: 4407049 Published · ppublish English Journal Article

Under-replication of ribosomal cistrons in polytene chromosomes of Rhynchosciara.

The Journal of cell biology ·Vol. 62 ·No. 1 ·1974-07-00 ·Pages 215-22

Gambarini AG, Lara FJ

Abstract

DNA preparations obtained from several tissues of Rhynchosciara americana and two related species, R. milleri and R. papaveroi, were hybridized to R. americana rRNA. The percentage of hybridization was found to be higher in tissues with low polyteny than in tissues with high polyteny, suggesting a relationship between the amount of rDNA and the tissue polyteny. This could be explained by under-replication of ribosomal cistrons in polytene cells, such as those from the salivary gland. Only slight tissue-dependent changes in the percentages of hybridization can be observed in heterologous hybridization using Xenopus laevis rRNA. The possibility that these experiments could not detect differences in the amount of ribosomal cistrons among tissues is discussed. The female:male ratio for the percentages of hybridization in the salivary gland of R. americana agrees with the results obtained by in situ hybridization experiments (16, 17) which have shown that the rRNA cistrons are distributed among chromosomes other than chromosome X.

MeSH Terms
Adipose Tissue/cytology,metabolism Animals Centrifugation, Density Gradient Chromosomes/metabolism DNA Replication Female Genes Insecta/cytology,metabolism Larva Male Metamorphosis, Biological Nucleic Acid Hybridization Organ Specificity Orotic Acid/metabolism Ovary/cytology,metabolism RNA, Ribosomal/biosynthesis Salivary Glands/cytology,metabolism Species Specificity Time Factors Tritium Uridine/metabolism
Chemicals
RNA, Ribosomal Tritium Orotic Acid Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gambarini A G
Lara F J
References (24)
24 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.62.1.215
Published
1974-07-00
Pages
215-22
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109172
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.62.1.215 PMC Full Text View journal details
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