Home LiteratureArticle Details
PMID: 4417406 Published · ppublish English Journal Article

Poly(A) polymerase from vaccinia virus-infected cells. I. Partial purification and characterization.

Journal of virology ·Vol. 14 ·No. 4 ·1974-10-00 ·Pages 715-23

Brakel C, Kates JR

Abstract

Poly(A) polymerase activity is induced during vaccinia virus infection of HeLa cells. The enzyme is maximally induced at 3.5 h postinfection. Partial purification frees the preparation of RNase activity and RNA polymerase activity. ATP is the substrate for poly(A) synthesis. A small amount of poly(A) is produced from added adenosine diphosphate due to the production of ATP by an adenylate kinase present in the preparation. The incorporation of ATP into poly(A) is dependent on divalent cations (Mg(2+) or Mn(2+)) and is not inhibited by UTP, CTP, or GTP. Poly(U) stimulates ATP incorporation; poly(A) and poly(C) have little effect on ATP incorporation, and poly(dT) is extremely inhibitory. RNA prepared from HeLa cells and from the partially purified poly(A) polymerase (the enzyme preparation contains endogenous RNA [Brakel and Kates]) stimulates ATP incorporation by poly(A) polymerase which was subjected to DEAE-cellulose chromatography. RNase's, pancreatic and T(1), inhibit the production of poly(A). DNase has little effect. Poly(U) is able to stimulate poly(A) production in the presence of T(1) RNase.

MeSH Terms
Adenine Nucleotides Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Ammonium Sulfate Carbon Radioisotopes Cell-Free System Chemical Precipitation Chromatography DNA-Directed RNA Polymerases/metabolism Dactinomycin/pharmacology Enzyme Induction HeLa Cells/enzymology Humans Magnesium/pharmacology Manganese/pharmacology Mycotoxins/pharmacology Nucleotides/pharmacology Nucleotidyltransferases/isolation & purification,metabolism Oligonucleotides/pharmacology Polynucleotides/biosynthesis Polysaccharides RNA, Neoplasm/pharmacology Ribonucleases/pharmacology Time Factors Tritium Vaccinia virus/growth & development
Chemicals
Adenine Nucleotides Carbon Radioisotopes Mycotoxins Nucleotides Oligonucleotides Polynucleotides Polysaccharides RNA, Neoplasm Tritium Dactinomycin Manganese Adenosine Diphosphate Adenosine Triphosphate Nucleotidyltransferases DNA-Directed RNA Polymerases Ribonucleases Magnesium Ammonium Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brakel C
Kates J R
References (27)
27 references, click to expand
  1. Polyriboadenylate synthesis by nuclei from developing sea urchin embryos. I. Characterization of the ATP polymerase reaction.
    Biochim Biophys Acta. 1967 Jun 20;142(1):246-53 PMID: 4293711
  2. Polyriboadenylate synthesis by nuclei from developing sea urchin embryos. II. Polyriboadenylic acid priming of ATP polymerase.
    Biochim Biophys Acta. 1967 Jun 20;142(1):254-62 PMID: 6058133
  3. Synthesis and intracellular localization of vaccinia virus deoxyribonucleic acid-dependent ribonucleic acid polymerase.
    J Virol. 1968 Sep;2(9):894-900 PMID: 4235691
  4. Ribonucleic acid synthesis in vaccinia virus. II. Synthesis of polyriboadenylic acid.
    J Mol Biol. 1970 May 28;50(1):19-33 PMID: 5453355
  5. Properties of a polyriboadenylate polymerase isolated from yeast ribosomes.
    Biochemistry. 1971 Apr 27;10(9):1576-82 PMID: 5103999
  6. Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA.
    Science. 1971 Oct 29;174(4008):507-10 PMID: 5110429
  7. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9 PMID: 5315962
  8. An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1321-5 PMID: 5288381
  9. A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5 PMID: 5288382
  10. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40 PMID: 5288383
  11. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):417-21 PMID: 4501121
  12. The genome of RNA tumor viruses contains polyadenylic acid sequences.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):791-4 PMID: 4337237
  13. Polyadenylic acid sequences in the virion RNA of poliovirus and Eastern Equine Encephalitis virus.
    Science. 1972 May 5;176(4034):526-8 PMID: 4338097
  14. Polyadenylic acid at the 3'-terminus of poliovirus RNA.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1877-82 PMID: 4340162
  15. Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid.
    J Virol. 1972 Oct;10(4):683-8 PMID: 4343546
  16. [Polyadenylate synthetase from mouse ascites cells. I. Purification and characterization of the enzyme].
    Biochim Biophys Acta. 1972 Dec 22;287(3):438-47 PMID: 4641253
  17. [Polyadenylate synthetase from mouse ascites cells. II. Enzyme reaction].
    Biochim Biophys Acta. 1972 Dec 22;287(3):448-55 PMID: 4565734
  18. Terminal riboadenylate transferase: a polyadenylate polymerase from calf thymus gland.
    Biochem Biophys Res Commun. 1973 Feb 5;50(3):737-43 PMID: 4689074
  19. Polyadenylate synthesis by extracts from L cells infected with vesicular stomatitis virus.
    Nat New Biol. 1973 Jun 13;243(128):200-3 PMID: 4351755
  20. Polyadenylate polymerase from vaccinia virions.
    Nat New Biol. 1973 Sep 12;245(141):59-63 PMID: 4517048
  21. Terminal riboadenylate transferase: a poly A polymerase in purified vaccinia virus.
    J Virol. 1973 Aug;12(2):203-8 PMID: 4201186
  22. Mechanism of poly(A) synthesis by vaccinia virus.
    J Virol. 1974 Aug;14(2):214-24 PMID: 4847326
  23. Poly(A) polymerase from vaccinia virus-infected cells. II. Product and primer characterization.
    J Virol. 1974 Oct;14(4):724-32 PMID: 4417407
  24. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  25. Nature of a polynucleotide required for polyribonucleotide formation from adenosine triphosphate with an enzyme from thymus nuclei.
    J Biol Chem. 1962 Aug;237:2636-42 PMID: 13889231
  26. THE INTRACELLULAR UNCOATING OF POXVIRUS DNA. II. THE MOLECULAR BASIS OF THE UNCOATING PROCESS.
    J Mol Biol. 1964 Feb;8:277-88 PMID: 14126296
  27. Polynucleotide biosynthesis: formation of a sequence of adenylate units from adenosine triphosphate by an enzyme from thymus nuclei.
    J Biol Chem. 1960 Apr;235:1142-9 PMID: 13819354
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.4.715-723.1974
Published
1974-10-00
Pages
715-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355574
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.4.715-723.1974 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2