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PMID: 4419608 Published · ppublish English Journal Article

Proteins of the postsynaptic density.

The Journal of cell biology ·Vol. 63 ·No. 2 Pt 1 ·1974-11-00 ·Pages 456-65

Banker G, Churchill L, Cotman CW

Abstract

An analysis was made of the protein composition of a fraction of postsynaptic densities (PSDs) prepared from rat brain. Protein makes up 90% of the material in the PSD fraction. Two major polypeptide fractions are present, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The major polypeptide fraction has a molecular weight of 53,000, makes up about 45% of the PSD protein, and comigrates on gels with a major polypeptide of the synaptic plasma membrane. The other polypeptide band has a molecular weight of 97,000, accounts for 17% of the PSD protein, and is not a prominent constituent of other fractions. Six other polypeptides of higher molecular weight (100,000-180,000) are consistently present in small amounts (3-9% each). The PSD fraction contains slightly greater amounts of polar amino acids and proline than the synaptic plasma membrane fraction, but no amino acid is usually prominent. The PSD apparently consists of a structural matrix formed primarily by a single polypeptide or class of polypeptides of 53,000 molecular weight. Small amounts of other specialized proteins are contained within this matrix.

MeSH Terms
Amino Acids/analysis Animals Brain Chemistry Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Mathematics Molecular Weight Nerve Tissue Proteins/analysis Peptides/analysis Rats Sodium Dodecyl Sulfate Solubility Synapses/analysis
Chemicals
Amino Acids Nerve Tissue Proteins Peptides Sodium Dodecyl Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Banker G
Churchill L
Cotman C W
References (32)
32 references, click to expand
  1. Cytochemistry of synapses: selective staining for electron microscopy.
    Science. 1966 Dec 23;154(3756):1575-7 PMID: 5924927
  2. Isolation of lipid-free plasma membrane proteins by gel filtration on Sephadex LH-20 using 2-chloroethanol-water as solvent.
    Biochim Biophys Acta. 1967 May 2;135(2):371-4 PMID: 6037368
  3. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
    Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
  4. Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid.
    J Ultrastruct Res. 1968 Mar;22(5):361-75 PMID: 4173151
  5. Group-specific reagents in protein chemistry.
    Annu Rev Biochem. 1968;37:695-726 PMID: 4875724
  6. Reaction of proteins with glutaraldehyde.
    Arch Biochem Biophys. 1968 Jul;126(1):16-26 PMID: 4174905
  7. Isolation and characterization of insoluble proteins of the synaptic plasma membrane.
    Arch Biochem Biophys. 1968 Sep 10;126(3):821-37 PMID: 5686594
  8. The colchicine-binding protein of mammalian brain and its relation to microtubules.
    Biochemistry. 1968 Dec;7(12):4466-79 PMID: 5700666
  9. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  10. Unified theory for gel electrophoresis and gel filtration.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):970-7 PMID: 4191703
  11. Recent developments in chemical modification and sequential degradation of proteins.
    Adv Protein Chem. 1970;24:261-308 PMID: 4915249
  12. The cytochemistry of synaptic densities. I. An analysis of the bismuth lodide impregnation method.
    J Ultrastruct Res. 1971 Jan;34(1):103-22 PMID: 4099757
  13. Estimation of molecular radius, free mobility, and valence using polyacylamide gel electrophoresis.
    Anal Biochem. 1971 Mar;40(1):95-134 PMID: 5550151
  14. Effect of charge on the determination of molecular weight of proteins by gel electrophoresis in SDS.
    Biochem Biophys Res Commun. 1971 Mar 19;42(6):1117-21 PMID: 4927800
  15. Characterization of collagen peptides by sodium dodecylsulfate-polyacrylamide electrophoresis.
    Anal Biochem. 1971 Jun;41(2):510-6 PMID: 4326447
  16. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  17. Cyclic 3',5'-nucleotide phosphodiesterase: cytochemical localization in cerebral cortex.
    Science. 1971 Aug 20;173(3998):745-7 PMID: 4327990
  18. Polypeptide chains from human red blood cell membranes.
    J Biol Chem. 1971 Jul 25;246(14):4485-8 PMID: 4999065
  19. Heterogeneity of tubulin subunits.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2028-31 PMID: 5289362
  20. Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2273-7 PMID: 5289385
  21. Glycoproteins: isolation from cellmembranes with lithium diiodosalicylate.
    Science. 1971 Dec 17;174(4015):1247-8 PMID: 5133448
  22. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):390-5 PMID: 4334138
  23. Molecular weights of the polypeptide chains of synaptic plasma membranes.
    Brain Res. 1972 Jul 20;42(2):508-13 PMID: 5050181
  24. Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.
    J Biol Chem. 1972 Sep 25;247(18):5856-61 PMID: 4560419
  25. Isolation and structural studies on synaptic complexes from rat brain.
    J Cell Biol. 1972 Dec;55(3):696-711 PMID: 4656707
  26. Differences in membrane structure between excitatory and inhibitory synapses in the cerebellar cortex.
    J Comp Neurol. 1974 May 1;155(1):93-125 PMID: 4836065
  27. Localization and characterization of concanavalin A receptors in the synaptic cleft.
    J Cell Biol. 1974 Jul;62(1):236-42 PMID: 4407356
  28. Isolation of postsynaptic densities from rat brain.
    J Cell Biol. 1974 Nov;63(2 Pt 1):441-55 PMID: 4138148
  29. Anionic sites of human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles.
    J Cell Biol. 1973 Nov;59(2 Pt 1):395-406 PMID: 4141707
  30. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
    Methods Enzymol. 1972;26:3-27 PMID: 4680711
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.63.2.456
Published
1974-11-00
Pages
456-65
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110954
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.63.2.456 PMC Full Text View journal details
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