Microtubule-macrotubule transitions: intermediates after exposure to the mitotic inhibitor vinblastine.

Science (New York, N.Y.) ·Vol. 186 ·No. 4170 ·1974-12-27 ·Pages 1219-21

Warfield RK, Bouck GB

Abstract

Vinblastine treatment of microtubule protein or intact microtubules assembled in vitro produced bifilar rings and bifilar helices. Suspentsions of rings and helices were demonstrated to bind [(3)H]colchicine, a diagnostic property of microtubule protein. Macrotubules are suggested to consist of tightly coiled helices formed by longitudinal compacting of loosely coiled protofilament pair intermediates.

MeSH Terms

Animals Binding Sites Brain/ultrastructure Colchicine/metabolism In Vitro Techniques Macromolecular Substances Microscopy, Electron Microtubules/drug effects,ultrastructure Models, Structural Nerve Tissue Proteins/metabolism Protein Binding Protein Conformation Swine Tritium Vinblastine/pharmacology

Chemicals

Macromolecular Substances Nerve Tissue Proteins Tritium Vinblastine Colchicine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Warfield R K

Bouck G B

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.186.4170.1219

Published

1974-12-27

Pages

1219-21

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.186.4170.1219 View journal details

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Microtubule-macrotubule transitions: intermediates after exposure to the mitotic inhibitor vinblastine.

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