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PMID: 444210 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparison of endogenous and exogenous RNA primers of poly(U) polymerase in rat hepatic ribosomes.

The Biochemical journal ·Vol. 177 ·No. 3 ·1979-03-01 ·Pages 895-902

Hayashi TT, MacFarlane K

Abstract

The present work indicates that RNA primer requirements for poly(U) polymerase in the free ribosomes of the rat liver depend upon the degree of enzyme purification. The poly(U) polymerase activity obtained from a crude free ribosomal preparation was compared with the enzymic activity of a partially purified enzyme. After preliminary purification, the enzyme was fractionated by chromatography on Sephadex G-150 and CM-cellulose. Our results demonstrate the presence of several forms of poly(U) polymerase activities, some requiring exogenous RNA and others possessing their own endogenous primer RNA.

MeSH Terms
Animals Hydrolysis In Vitro Techniques Isoenzymes/isolation & purification,metabolism Liver/enzymology Male Nucleotidyltransferases/isolation & purification,metabolism Poly U RNA/metabolism Rats Ribosomes/enzymology
Chemicals
Isoenzymes Poly U RNA Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hayashi T T
MacFarlane K
References (24)
24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1770895
Published
1979-03-01
Pages
895-902
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186455
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1770895 PMC Full Text View journal details
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