The effect of systematic error on the accuracy of Michaelis constants and maximum velocities estimated by using the integrated Michaelis-Menten equation.

The Biochemical journal ·Vol. 143 ·No. 3 ·1974-12-00 ·Pages 779-81

Newman PF, Atkins GL, Nimmo IA

Abstract

Systematic errors in initial substrate concentration (s(0)), product concentration and reaction time give much larger errors in the Michaelis-Menten parameters unless s(0) is treated as an unknown parameter. These errors are difficult to detect because the fitted curve deviates little from the data. The effect of non-enzymic reaction is also examined.

MeSH Terms

Enzymes/metabolism Kinetics Mathematics Models, Biological

Chemicals

Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Newman P F

Atkins G L

Nimmo I A

References (4)

4 references, click to expand

The reliability of Michaelis constants and maximum velocities estimated by using the integrated Michaelis-Menten equation.
Biochem J. 1973 Dec;135(4):779-84 PMID: 4778274
Statistical estimations in enzyme kinetics.
Biochem J. 1961 Aug;80:324-32 PMID: 13785321
A comparison of two methods for fitting the integrated Michaelis-Menten equation.
Biochem J. 1974 Sep;141(3):913-4 PMID: 4463971
Statistical estimations in enzyme kinetics. The integrated Michaelis equation.
Eur J Biochem. 1974 Apr 1;43(2):377-8 PMID: 4838986

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1430779

Published

1974-12-00

Pages

779-81

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1168449

Subset

External Links

PubMed source DOI: 10.1042/bj1430779 PMC Full Text View journal details

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The effect of systematic error on the accuracy of Michaelis constants and maximum velocities estimated by using the integrated Michaelis-Menten equation.

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