Abstract
1. Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.
MeSH Terms
Centrifugation
Chemical Fractionation
Chromatin/analysis,isolation & purification
Chromatography, DEAE-Cellulose
Culture Techniques
DNA/metabolism
DNA-Directed RNA Polymerases/analysis
Plant Growth Regulators
Plants/enzymology
Protein Binding
Tissue Extracts
Tritium
Uracil Nucleotides
Chemicals
Chromatin
Plant Growth Regulators
Tissue Extracts
Uracil Nucleotides
Tritium
DNA
DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gore J R
Ingle J
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