Home LiteratureArticle Details
PMID: 4464848 Published · ppublish English Journal Article

Ribonucleic acid polymerase activities in Jerusalem artichoke tissue.

The Biochemical journal ·Vol. 143 ·No. 1 ·1974-10-00 ·Pages 107-113

Gore JR, Ingle J

Abstract

1. Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.

MeSH Terms
Centrifugation Chemical Fractionation Chromatin/analysis,isolation & purification Chromatography, DEAE-Cellulose Culture Techniques DNA/metabolism DNA-Directed RNA Polymerases/analysis Plant Growth Regulators Plants/enzymology Protein Binding Tissue Extracts Tritium Uracil Nucleotides
Chemicals
Chromatin Plant Growth Regulators Tissue Extracts Uracil Nucleotides Tritium DNA DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gore J R
Ingle J
References (16)
16 references, click to expand
  1. The DNA-directed RNA polymerases of soybean.
    Biochim Biophys Acta. 1973 Jan 19;294(2):227-35 PMID: 4734933
  2. Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.
    Nature. 1969 Oct 18;224(5216):234-7 PMID: 5344598
  3. The Influence of Auxin and Ethylene on Chromatin-directed Ribonucleic Acid Synthesis in Soybean Hypocotyl.
    Plant Physiol. 1970 Jan;45(1):41-5 PMID: 16657277
  4. Chromatin- and nuclei-directed ribonucleic Acid synthesis in sugar beet root.
    Plant Physiol. 1971 Feb;47(2):262-8 PMID: 16657606
  5. Enzymatic synthesis of RNA.
    Biochem Biophys Res Commun. 1960 Dec;3:689-94 PMID: 13716400
  6. Wheat leaf RNA polymerases. I. Partial purification and characterization of nuclear, chloroplast and soluble DNA-dependent enzymes.
    Arch Biochem Biophys. 1971 Oct;146(2):635-48 PMID: 5114923
  7. RNA polymerases of maize: nuclear RNA polymerases.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2647-51 PMID: 5288239
  8. RNA polymerase from eukaryotic cells. Isolation and purification of enzymes and factors from chromatin of coconut nuclei.
    Eur J Biochem. 1972 Feb;25(3):463-70 PMID: 4339642
  9. Increased ribonucleic Acid polymerase activity associated with chromatin from internodes of dwarf pea plants treated with gibberellic Acid.
    Plant Physiol. 1970 Aug;46(2):221-3 PMID: 16657438
  10. RNA polymerase of HeLa cells.
    Biochim Biophys Acta. 1967 Sep 26;145(2):272-83 PMID: 6070748
  11. Synthesis and Stability of Chloroplast Ribosomal-RNA's.
    Plant Physiol. 1968 Sep;43(9):1448-54 PMID: 16656936
  12. Isolation, purification and characterization of RNA polymerases from wheat germ.
    Biochim Biophys Acta. 1973 Aug 10;319(1):48-54 PMID: 4739046
  13. Increased DNA template and RNA polymerase associated with the breaking of seed dormancy.
    Plant Physiol. 1968 Oct;43(10):1734-6 PMID: 16656964
  14. Enhancement by 2,4-dichlorophenoxyacetic acid of chromatin RNA polymerase in soybean hypocotyl tissue.
    Biochim Biophys Acta. 1968 Nov 20;169(1):35-43 PMID: 4302411
  15. Wheat leaf RNA polymerases. II. Kinetic characterization and template specificities of nuclear, chloroplast, and soluble enzymes.
    Arch Biochem Biophys. 1971 Oct;146(2):649-57 PMID: 5114924
  16. Ribonucleic Acid Synthesis by Cucumber Chromatin: Developmental and Hormone-induced Changes.
    Plant Physiol. 1970 Oct;46(4):581-5 PMID: 16657509
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1974-10-00
Pages
107-113
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168358
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]