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PMID: 4490525 Published · ppublish English Journal Article

Absence of circular plasmid deoxyribonucleic acid attributable to a genetic determinant for methicillin resistance in Staphylococcus aureus.

Journal of bacteriology ·Vol. 116 ·No. 2 ·1973-11-00 ·Pages 771-7

Stiffler PW, Sweeney HM, Cohen S

Abstract

Plasmid deoxyribonucleic acid was not detected by centrifugal analysis of lysates of penicillinase-negative strains of Staphylococcus aureus harboring a determinant of methicillin resistance derived from strain Villaluz. When these strains contained a penicillinase plasmid, the plasmid deoxyribonucleic acid of methicillin-resistant and methicillin-susceptible strains was indistinguishable by the methods employed. The results indicate that the genetic determinant for methicillin resistance in the strains examined was not associated with a circular plasmid comparable to those that have been shown to determine resistance to benzylpenicillin, tetracycline, and chloramphenicol in S. aureus.

MeSH Terms
Centrifugation, Density Gradient Cesium DNA, Bacterial/analysis DNA, Circular/analysis Dialysis Ethidium Extrachromosomal Inheritance Genetic Code Methicillin/pharmacology Molecular Weight Penicillin Resistance Staphylococcus/analysis,drug effects Sucrose Thymidine/metabolism Tritium
Chemicals
DNA, Bacterial DNA, Circular Tritium Cesium Sucrose Ethidium Methicillin Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stiffler P W
Sweeney H M
Cohen S
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.2.771-777.1973
Published
1973-11-00
Pages
771-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285444
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.2.771-777.1973 PMC Full Text View journal details
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