Characterization of messenger ribonucleoprotein and messenger RNA from KB cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 3 ·1972-03-00 ·Pages 681-5

Kumar A, Lindberg U

Abstract

Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 x 10(6).

MeSH Terms

Cell Fractionation Cells, Cultured Centrifugation, Density Gradient Electrophoresis, Starch Gel Humans Molecular Weight Nucleoproteins/analysis RNA, Messenger/analysis RNA, Ribosomal/analysis Ribosomes/analysis Tritium Uridine/metabolism

Chemicals

Nucleoproteins RNA, Messenger RNA, Ribosomal Tritium Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kumar A

Lindberg U

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.3.681

Published

1972-03-00

Pages

681-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426534

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.3.681 PMC Full Text View journal details

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Characterization of messenger ribonucleoprotein and messenger RNA from KB cells.

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