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PMID: 4502939 Published · ppublish English Journal Article

Complete amino-acid sequence of calf-thymus histone 3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 4 ·1972-04-00 ·Pages 882-4

DeLange RJ, Hooper JA, Smith EL

Abstract

Calf-thymus histone III is a single polypeptide chain of 135 residues (combined molecular weight of 15,324) with alanine at both the amino and carboxyl ends. The NH(2)-terminal region (Residues 1-53) of histone III is strongly basic (net charge of +18) and contains lysines-14 and -23, which are epsilon-N-acetylated in a fraction of the molecules, as well as lysines-9 and -27, which are partially epsilon-N-methylated. The COOH-terminal region (Residues 54-135) is only slightly basic (net charge of +4), contains most of the hydrophobic residues, and has a 29-residue sequence that lacks a basic residue. The two cysteines are in the nonbasic region at positions 96 and 110. A few sequence similarities of calf-thymus histone III with other histones have been noted.

MeSH Terms
Acylation Amino Acid Sequence Amino Acids/analysis Animals Cattle Histones/analysis Iodoacetates Methylation Oxidation-Reduction Thymus Gland/analysis
Chemicals
Amino Acids Histones Iodoacetates
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DeLange R J
Hooper J A
Smith E L
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.4.882
Published
1972-04-00
Pages
882-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426586
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.4.882 PMC Full Text View journal details
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